VETERINARY MEDICINE 583 



lnfecte<l with hog cholera some peculiarities in the red blood corpuscles were 

 observed. 



Wheu red blood corpuscles were studied iu a fresh (unfixed and unstained) 

 state they were found to throw out, from their margins, processes from the 

 cytoplasm as active bodies of various bizarre forms which, in part, form the 

 plasma granules seen by dark ground illumination. A more common feature 

 of pigs' blood, and one which distinguishes it from any other animal species, is 

 the occuri-ence within the area of the red cell of refringent motile bodies, 

 which, with the highest of magnifications, show a certain degree of uniformity 

 in shape and ai*e apparently intracellular. They usually lie near the margin 

 of the cell, but in their migrations they may alternately be above or below 

 the focus, but never beyond the margin of the cell. Although first observed in 

 normal pigs, they were noted in much increased quantities in the blood of 

 hogs affected with cholera. The motility is inhibited by cold but goes on at 

 ordinary room temperature, i. e., 70° or upward. " The motility of these 

 endoglobular bodies is not to be compared with that of bacteria, nor can it l)o 

 I)roi>erly described as ameboid, nor yet as molecular." 



" Omitting the smallest bodies too minute to allow recognition of their shape 

 these bodies occur in three distinct forms: (1) As roimd or short oval slightly 

 biconcave flattened disks; (2) as long oval or rice grain-like bodies; (3) as 

 crescentic or horseshoe-like forms. All of these forms may be found in the sarao 

 blood sample. Usually the disk form is most abundant. It also exhibits the most 

 active motility and the greatest diversity in size." The crescentic or horseshoe 

 shaped forms are the most sluggish in motion. 



" The erythrocytes in which these bodies occur may be of normal size or slightly 

 larger or smaller than the average and are not commonly otherwise distorted. 

 Most frequently they occur singly in the cell, but doubles are nearly as common 

 and six or eight may be found. Those cells which carry such numbers are 

 generally smaller than the average, and I have only found them in cholera- 

 infected blood." The cells in which these motile bodies are present are not 

 obviously deficient in hemoglobin. 



" Between the cells in pigs' blood and more numerously in infected blood, 

 a variety of minute quivering or dancing bodies may be seen, the origin and 

 nature of which I do not know." These may be offshoots of erythrocytes and 

 are increased some hours after collecting the blood. 



"In diseased blood (cholera infected) the Intercellular or plasma granules 

 are usually more numerous than in normal blood. In all pigs' blood they are 

 greatly more numerous than in the blood of man." Spirochetes as observed 

 by King (E. S. R., 30, p. 383) were not found. 



In stained specimens of pigs' blood small round solidly stained bodies were 

 seen within the erythrocytes. Only one of these coccoid bodies is usually 

 present and it is eccentrically placed in the blood cell. " With methylene blue 

 or Jenner's stain they are colored blue, with Giemsa, a dark ruby red. As to 

 the erythrocytes, in which they occur, they are, with the exception to be noted, 

 not otherwise abnormal. In the blood of some infected animals in which 

 marked anisocytosls was present with diminished red cell count, most of these 

 bodies would be found within enlarged cells (megalocytes) with deficient 

 hemoglobin. In the same preparation, however, bodies would be seen within 

 microcytes or cells of normal size. Unusual numbers of these bodies in any 

 specimen were found quite generally associated with such abnormalities in the 

 erythrocytes. Erythroblasts may be found in preparations from the blood of 

 normal or noninfected pigs. They are often more abundant in the blood of 

 cholera-infected animals, especially at a late stage of a chronic form of the 



