480 EXPERIMENT STATION RECORD. [Vol.37 



removing by centrifugalization. A second washing was carried out, and the 

 bacilli remaining were extracted by heating for one hour with 5 per cent 

 glycerin In distilled water, then removed by centrifugalization or passing the 

 liquid through a Berkefeld filter. 



These preparations were approximately of the same strength, 10 cc. ot the 

 liquid representing the extract from 1 gm. of the wet bacilli. A concentrated 

 Johnin used in the conjunctival and intracutaneous tests was prepared by 

 evaporating the ordinary Johnin to one-fifth of its original volume or by extract- 

 ing washed bacilli with 5 per cent glycerin in water in the proportion of 2 cc. 

 to 1 gm. of the wet bacilli. 



Subcutaneous, intracutaneous, and conjunctival tests of animals experimen- 

 tally infected with the disease and animals experimentally infected with numan. 

 avian, and bovine tubercle bacilli are reported in detail. The .Tohnin pro- 

 duced distinct temperature reactions in animals affected with Johne's disease. 

 Positive results were obtained by the intracutaneous procedure, but the appli- 

 cation of tlie Johnin to the conjunctiva appeared to be incapable of produc- 

 ing distinct reactions. Reactions with the Johnin were also obtained in young 

 animals infected with human, avian, or bovine tubercle bacilli. 



A new Italian method for antirabic treatment, C. Fekmi (Ann. Ig. Uper., 

 n. «er., 26 (1916), Sup., pp. 164). — This volume describes the results of an ex- 

 perimental investigation. Part 1 consists of a description of the new method 

 and its evolution ; part 2 gives a comparison of the efficacy of the new method 

 with other methods of antirabic treatment ; and part 3 shows the duratiun of 

 the immunizing power of the vaccine and serum vaccine and of the Immuniz- 

 ing and neutralizing power of the new antirabic .serum. 



In the procedure a vaccine consi.sting of a 5 per cent emulsion of the virus, 

 an antirabic serum obtained from the horse, and a serum vaccine consisting 

 of one part of the antiserum and two of the vaccine, together with 1 per cent 

 phenol, are used. The method of treatment and detailed tabulated data are 

 described and discussod. 



Rinderpest. — Preparation of antiserum, A. W. Shilston (Agr. Research 

 Inst. Pusa Bui. 64 (1916), pp. lH). — Experiments to determine the potency of 

 sera taken 8, 12, and 10 days after the injection of virus, and to compare the 

 results with those given by sera taken 15 and 17 days after Injection, are 

 reported. 



The results indicate that the immune bodies are present in full amount in 

 the serum 8 days after the injection of the virulent blood and citrate solu- 

 tion mixture. " By taking three bleedings at the rate of 6 cc. per pound body 

 weight on the eighth, twelfth, and sixteenth days after injection a mixed 

 serum was obtained of equal (hill bulls) or increased (buffaloes) potency to 

 that obtained by taking two bleedings 15 and 17 days after injection at the rate 

 of G cc. and 8 cc. per pound body weight, respectively. . . . The actual yield 

 of serum after each injection was Increased from 6.79 cc. per pound botly 

 weight by the two bleeilings system to 9.6 cc. by the three bleedings system or 

 an additional 2.81 cc. of serum per {wund body weight ; an increase of 41.4 

 per cent on the former output." 



The saving of cost in the production of a large amount of serum by tlie new 

 procedure is noted. 



The piroplasmoses and means for their prevention and control, G. AiJis- 

 8ANDUIN1 (Ann. Ig. [Rome], 27 (1917), No. 2, pp. 100-110; abs. in Rev. Appl. 

 Ent., Ser. D, 5 (1917), No. 5, p. 71). — A brief review of the present status of 

 the knowledge of piroplasmoses and babesiases and their control. Including an 

 account of the ticks implicated in their transmission. 



