384 EXPERIMENT STATION BECORD. [Vol.35 



sulphate retarded the injury. It is concluded that acidosis played only a small 

 part, if any, in the injury produced. 



In a restricted ration, such as used in one series, pigs were seriously in- 

 jured or killed within four to six weeks by eating digester tankage in amounts 

 of nitrogen equivalent to that in a provisional lethal dose of cotton-seed meal, 

 which was approximately 15 gm. nitrogen daily for each pig. This would 

 indicate that, if cotton-seed meal is fed in a restricted ration and in large 

 quantities, the ration may injure and kill pigs, even though it should contain 

 no specific toxic substance." 



On the intermediate host of the lung distome, Paragonimus westermani, 

 S. YosHiDA {Jour. Parasitology, 2 {1916), No. 8, pp. 111-118, pi. 1). — "In For- 

 mosa Nakagawa found the encysted larvae in two fresh-water crabs and ex- 

 perimentally proved that they grew up to the lung distomes. The two crabs 

 were identified by A. Terao as follows: Potamon {Oeothelphusa) obtusipes [P. 

 {Geothelphusa) dehaanii']. Nakagawa added that a fresh-water crab {Erio- 

 cheir japonicus) will also probably prove to be the intermediate host. 



" I have experimentally proved that the encysted larvae of this worm are 

 found in three species of fresh-water crabs from various districts of Japan 

 proper. They are identified as follows: P. dehaanii, Sesarma dehaanii, and 

 E. japonicus." 



Are sarcosporidia aberrant forms of cnidosporidia of invertebrates P B. 

 Galli-Valerio {Jour. Parasitology, 2 {1916), No. 3, pp. 126-128) .—'' The obser- 

 vations of Plana and Galli-Valerio to the effect that spores of sarcosporidia 

 produce amebic bodies in cultures more closely relate the sarcosporidia to the 

 cnidosporidia. If true that sarcosporidia are only aberrant forms of neo- 

 sporidia of invertebrates, then the hypothesis of Darling becomes more prob- 

 able." 



The preparation of tetanus antitoxin, E. H. Ruedigee {Philippine Jour. Sci., 

 Sect. B, 10 {1915), No. 1, pp. 31-63, figs. 85).— From the results obtained, the 

 authors conclude that " a suitable strain of the bacillus of tetanus will usually 

 produce potent toxin when grown in nearly neutral glucose broth under 

 liydrogen. The acidity of the broth will rise to more than two per cent 

 normal acid, and it should be neutralized with sodium hydrate before it is 

 injected into the horse. Potent tetanus toxin was obtained by the method 

 described by Ivan Hall.* By this method the acid is continuously neutralized 

 by the magnesium carbonate present. 



" Horses differ gi-eatly in the power of producing tetanus antitoxin." Of 

 eight horses reported on, one produced 150 units, two 300 units, one 350 units, 

 cue 400 units, and three 500 or more units of tetanus antitoxin per cubic 

 centimeter of serum. The antitoxin curve reached its highest mark in from 

 six to nine months after the beginning of immunization. 



" The injection of large doses of toxin is not indicated. The dosage should 

 be such that the horse does not appreciably lose in weight." 



The conjunctival tuberculin reaction, Besnoit and Cuilli6 {Rev. 06n. M6d. 

 r6t., 25 {1916), No. 289, pp. 9-17, fig. i).— The authors have found the con- 

 junctival tuberculin reaction for the detection of bovine tuberculosis of great 

 diagnostic value and equal to the classical subcutaneous reaction. The tech- 

 nique is simple and rapid and has the advantage of not causing great rises 

 in temperature. The possibilities of diagnostic error are considerably reduced. 

 The procedure is valuable both in investigational and in practical routine work. 

 The authors believe that it should be substituted for the subcutaneous method, 

 the latter being reserved exclusively for the control of uncertain cases. 



« Univ. Cal. Pubs., Path.. 1913, No. 2, p. 98, 



