284 EXPEKIMENT STATION" KECORD. 



above the ground : 0.3, 0.5, 0.75, 1, 1.3, and 1.6 meters. A thermometer was also 

 placed between the manure and the straw. After the whole of the dung had 

 been packed a layer of straw 0.1 meter thick was placed on the top and this 

 was covered with a similar layer of earth. The heap was left for 8 days during 

 which time the temperature varied from 6 to 14° and no rain fell. The manure 

 was then found to be well-rotted on the surface and felt quite warm. Upon re- 

 moval of the thermometers it was found that the temperature in various parts 

 of the heap varied from 55.5 to 75°, the highest temperatures beiug registered by 

 the thermometers in the lower layers. 



Young pigs which were inoculated with 1 cc. of a salt solution in which the 

 feet were crushed failed to become infected with foot-and-mouth disease. Full- 

 grown pigs were inoculated with from 12 to 15 cc. of the diluted lymph con- 

 tained in the tubes and not a single case became ill. The experiments indicate 

 that the whole of the virus was destroyed. 



The influence of the subcutaneous mallein test upon the examination of 

 the blood for diagnostic purposes, A. Marcis (AUatorvosi Lapok, 35 {1912), 

 Nos. 42, pp. 495-498, fig. 1; 43, pp. 508-511, fig. 1; abs. in Berlin. Tierarztl. 

 Wchnschr., 29 {1913), No. S, pp. 136, 137).— In the blood of horses treated with 

 mallein (subcutaneously), specific precipitins, agglutinins, and complement fix- 

 ing amboceptors are produced. These are also noted in horses infected with the 

 Bacillus mallei, and consequently blood examinations made after subcutaneous 

 malleinization are without value. 



After giving mallein subcutaneously, precipitins are first noted (in about 3 

 days), then the agglutinins (in from 5 to 7 days), and finally the complement 

 fixing substances (6 to 10 days). They begin to vanish in about 3 months, and 

 in practically the same order as they appeared. 



Diagnostic blood examinations can only be of value 3 months after subcuta- 

 neous malleinization. 



The laboratory diagnosis of glanders, E. M. Wade {Jour. Infect. Diseases, 

 12 {1913), No. 1, pp. 7-14). — "The diagnosis of glanders by the complement 

 fixation test supplemented by the agglutination test on all negative sera is 

 deemed the quickest and most reliable test at the present time. 



" Glanders antigen prepared without shaking but filtered through a Berkefeld 

 candle gives reliable results and yields a more stable product. 



" Normal horse serum varies widely in its agglutinating power, therefore a 

 weak positive should be considered in conjunction with the complement fixation 

 test or with clinical symptoms. 



"Antigens and agglutination fluids should be prepared from several strains of 

 Bacillus mallei. 



" While a positive result from guinea pig inoculation is conclusive evidence of 

 the presence of glanders, failure of the pigs to develop lesions is not proof of its 

 absence." 



A note in regard to the pathologic anatomy of chronic diarrhea in bovines 

 (enteritis chronica bovis paratuberculosa, Johne's disease), G. Moussu and 

 Faroy {Compt. Rend. 8oc. Biol. [Paris], 70 {1911), Nos. 21, pp. 938-9.'i0; 22, pp. 

 982-984; abs. in Bui. Inst. Pasteur, 9 {1911), No. 17, p. 761). — This disease is 

 not considered to be what is usually understood as pseudo-tuberculosis, nor is it 

 human, bovine, or avian tuberculosis. Giving cultures to chickens per os does 

 not confer a tuberculosis of the avian type, nor does the diseased animal give 

 any of the various tuberculin reactions. The patho-anatomical findings are 

 given in detail. 



In regard to the present status of vaccination against coast fever, K. 

 WoLFEL {Ztschr. Infektionskrank. u. Hyg. Haustiere, 12 {1912), No. 3, pp. 

 247-255). — This is a critical discussion and description of Thaller's vaccination 



