384 EXPERIMENT STATION RECORD. 



the discharge from the lungs or uterus is injected into guinea pigs without 

 previous treatment, but milk must be treated with 0.5 per cent of boric acid if 

 it is not used at once for injection. Where other organisms are present in the 

 discharge, it is advisable to subject the suspected or pathologic material to the 

 action of 5 per cent antiformin solution for from 2 to 3 hours. The antiformin 

 must then be removed, and the material is centrifuged and washed only once. 



For detecting open tuberculosis in bovines, the methods of Miiller, Wiemann, 

 Jonske, and Scharr and Opalka are deemed valuable. 



The detection of the tubercle bacillus in the feces of bovines, H. Thieein- 

 GER (Arb. E. Gsndhtsamt., J,3 {WIS), No. 4, PP. 5f,5-606).— As a result of mak- 

 ing some examinations of feces, it was found that the usual microscopic method 

 is not satisfactory for the detection of the presence of the tubercle bacillus. 

 The most certain method is the animal experiment. The following procedure is 

 recommended : 



A 30 gm. sample of the feces is mixed in an Erlenmeyer flask with 55 cc. of 

 sterile water and 15 cc. of antiformin until a uniform mixture free from 

 lumps is obtained. The mixture is then allowed to stand for 3 to 4 hours at room 

 temperature, and during this period it is shaken occasionally. After this time, 

 the mixture is shaken in order to get a uniform suspension. 70 cc. distributed 

 in several centrifuge tubes, and centrifuged in a high-speed electrical apparatus 

 for 5 minutes, or in a water power centrifuge of 1,400 revolutions per minute 

 for 20 minutes. The supernatant fluid is then jwured off, and the residue sus- 

 pended in sterile water, regulating the amount of water with reference to the 

 number of guinea pigs to be inoculated. After filtering through 2 layers of 

 gauze, stirring constantly during the filtration process, and finally kneading 

 the residue upon the gauze with a sterilized potato knife, the filtrate is in- 

 jected in 2 to 3 cc. doses subcutaneously into guinea pigs. 



Numerous kinds of pathogenic and nonpathogenic bacteria were c-ompared in 

 the test. 



Beport of the commission appointed by the ministry of agriculture, 

 Belgium., for the purpose of determining the efficiency of Heymans' method 

 for vaccinating against bovine tuberculosis {Ann. MM. V6t., 61 {1912), No. 8-9, 

 pp. 417-^52). — The plan of the commission was to make cohabitation tests, inges- 

 tion tests, and inoculation tests with vaccinated and unvaccinated animals, and 

 vaccinations on a certain number of farms in which tuberculosis had been 

 previously demonstrated to be present. 



The commission reports that the method is of no practical value for vacci- 

 nating against tuberculosis. The data as reported are tabulated. 



The treatment of bovine tuberculosis with Tuberculosan (Burow), H. 

 Peelich {Berlin. TierdrztJ. Wclmschr., 29 {1913), Nos. 7, pp. 116, 117; 8, pp. 

 133-135). — In these experiments 39 bovines were treated with Tuberculosan 

 (E. S. R., 26, pp. 680, 681). The animals were divided into 3 groups, namely, 

 animals pronounced tubercular on the basis of the tuberculin test, animals in 

 the first stage of tuberculosis, and advanced tubercular subjects. The results 

 obtained in general were satisfactory, thus confirming the findings of Burow. 



The question of tick eradication, P. F. Bahnsen {Dept. Agr. (hi.. Bui. 6— 

 S. v., 2. ed. [1913], pp. 29). — This bulletin consists of a series of questions and 

 answers relating to the cattle tick and its eradication in Georgia. 



Cooperia oncophora in diarrheic calves, A. W. N. Pillees {Vet. Rec, 25 

 (1912), No. 1272, pp. 305, 306). — The author records the occurrence of a disease 

 in 9 calves, apparently caused by this parasite in the walls of the small 

 intestines. 



Hog cholera, B. M. Bolton (Maryland SU. Bui. 17 Jf, pp. 1S5-169, figs. 6). — 

 The author discusses the history of this disease, its nature, contributing causes. 



