480 EXPERIMENT STATION RECORD. 



a case in a 5-year-old cavalry mare in which the disease was not recognized 

 for a long time, but was finally detected by a tuberculin injection and the 

 ophthalmo reaction. The symptoms, which were unusual, are described in 

 detail. 



About the detection of tubercle bacilli in the circulating blood, and the 

 practical significance of the same, H. Mammen ( Ueber den Nachwcis von 

 Tuhcrkelhacillen im strdmenden Blute und seine praktisclie Bedeutung. Inaug. 

 Diss., Univ. Giessen, 1911, pp. SJfj abs. in Hyg. Rundschau, 22 {1912), No. 16, p. 

 lOJtl). — Among 15 cases (6 cows, 6 oxen, a horse, a calf, and a guinea pig) 

 the tubercle bacillus was noted 12 times in the circulating blood. This was 

 not only in cases of advanced tuberculosis but also in instances where the 

 disease was hardly clinically noticeable; consequently, it is believed that the 

 value of detecting the presence of tubercle bacilli in the blood must not be 

 underestimated. The combined antiformin-ligroin method is preferred for this 

 purpose. 



Investigations in regard to the value of the alcohol test for detecting the 

 milk of diseased animals, K. Metzgeb {Untersuchungen Uber die AlkohoJprobe 

 bei Milch von kranken KUhen. Inaug. Diss., Univ. Stuttgart, 1912; abs. in 

 Milchw. Zentbl., 42 (1913), No. 1, pp. 15, 16).— The value of the alcohol test 

 was studied in connection with the acidity of milk coming from 18 cases of 

 tuberculosis, 26 cases of indigestion, 17 cases in which the genital oi'gans were 

 affected, and 9 cases of miscellaneous diseases. 



The results obtained varied, consequently the test is not deemed one which 

 can be relied upon for general diagnostic purposes. An exception, however, is 

 supposed to be made in diagnosing imi^euding abortion. A negative finding in 

 these cases points with certainty to the fact that abortion will not take place 

 within a certain time. 



About the action of tuberculin on the nontubercular guinea pig, and the 

 course of tuberculosis in the guinea pig pretreated v/ith tuberculin, F. Klop- 

 STocK {Ztschr. Expt. Path. u. Ther., IS {1913), No. 1. pp. 56-7i).— Repeated 

 injections with old tuberculin in an initial dose of from 0.1 to 5 cc. and up to a 

 dose of 26 cc. for the entire period, with intervals between injections of from 

 i to 4 weeks, produced no appreciable reaction in 15 out of 21 guinea pigs. In 

 the remaining animals it was lethal. Three of the dead animals had a slight 

 intestinal catarrh and showed a strong injection of the serosa of the lower 

 intestine. Two other animals died as a result of peritonitis. 



Pretreating animals with tuberculin for months did not seem to increase their 

 resistance to an experimental infection with the tubercle bacillus. The hyper- 

 susceptibility toward tuberculin injections is, however, reduced, as 4 out of 6 

 animals at the height of tuberculosis were able to withstand the effects of 1 cc. 

 of old tuberculin. Repeated injections of tuberculin into sound guinea pigs 

 did not stimulate the formation of antibodies. Animals pretreated with tuber- 

 culin and then rendered tuberculous did not show antibody formation, as was 

 evidenced by the complement fixation test. 



Investigations in regard to vaccinations against bovine tuberculosis with 

 laboratory animals (rabbits, guinea pigs), Bruschettini {Genibl. Bakt. 

 [etc.^, 1. Abt., Orig., 68 {1913), No. 3-Jf, pp. 337-S//2).— Comparative tests were 

 made on a number of rabbits and guinea pigs with the following vaccines : 

 (a) Tubercle bacilli grown on glycerin-bovine bile-potato; (bt bacilli with the 

 fat extracted; (c) bacilli treated with an alkaline menstrum at 60° C. : 

 (d) bacilli extracted with fat solvents in the cold and then treated at 60° with 

 an alkaline solution; (e) bacilli treated with chloroform at 40° and then in 

 vivo with leucocytes (according to the author's method). 



