206 EXPERIMENT STATION RECORD. [Vol.43 



into a capillary pipette and brought into a 100 cc. flask in which has been 

 placed 10 cc. of 1 per cent phosphoric acid. The flask is connected with a 

 detachable twice-bent distillation tube which is blown out into a bulb just 

 below the two bends and is drawn out at the end into a small opening about 

 0.2.1 ram. across. The receiver is a test tul)e 150 mm. by 15 mm., wliich is 

 inclosed by a cooler, allowing a free circulation of water. In the receiving test 

 tube are placed 3 cc. of n/2 NaOH and 2 cc. of n/200 iodin. The distillation is 

 carried on for 100 seconds after the solution begins to boil. After the tube 

 has stood for at least 3 minutes 3.5 cc. of N/2n2i?04 is added to the distillate, 

 the solution stirred, 2 drops of 1 per cent starch solution added, and the titra- 

 tion carried out with n/200 sodium thiosulphate. A blank test is conducted 

 in the same manner, and the titer of the iodin solution is determined by the 

 difference in the amounts of thiosulphate used in the two tests. 



The method, the details for which are given in full, is considered to be of 

 sufficient accuracy for the determination of acetone in pathological cases such 

 as diabetes, but not of sufficient delicacy for estimations of the acetone per- 

 centage in normal blood. 



The determination of albumin in urine, O. Mayek {Ztschr. Anahjt. Chein., 

 58 {1919), No. 8, pp. 337-8^6). — The author makes use of a ring test to deter- 

 mine albumin in urine quantitatively as well as qualitatively. The reagent 

 used is made by dissolving 10 gm. of mercuric chlorid, 25 gm. of citric acid, 

 and 65 gra. of pure sodium chlorid in 500 gm. of hot water, and filtering after 

 allowing it to stand for several days. To 5 cc. of this reagent in a conical glass 

 receptacle placed in a slanting position is added from a fine pipette an equal 

 amount of urine, and the time for the appearance of a white ring at the 

 juncture of the two liquids noted. If the concentration of the protein is 

 0.001 per cent, the ring appears in from two to three minutes from the time 

 the urine is added. Should the ring appear in less time, trial dilutions of 

 the urine are made until the ring appears within the given time. The con- 

 centration of tlie albumin in the urine can then be determined from the dilution 

 required. 



Decomposition of hydrogen peroxid by the microorganisms isolated from 

 pasteurized milk, M. Fouassier {Conipt. Rend. Acad. Sci. [Pom], 170 {1920), 

 No. 2, pp. lJf5-l-'i7). — ^Attention is called to the fact that in samples of pasteur- 

 ized milk to which hydrogen peroxid has been added immediately after pas- 

 teurization the peroxid disappears after some hours but before the milk shows 

 any traces of acidity. Experimental evidence is given which indicates that 

 this is caused by the presence in the milk of some spore-forming microorganisms 

 which have resisted pasteui'ization. 



The differentiation of agricultural seeds and feeding stuffs by means of 

 serum reactions, J. Becker {FuhUnrfs Laudw. Ztg., 67 {1918), No. 5-6, pp. 

 114-120; abs. in Chem. Abs., 14 {1920), No. 5, p. 552). — A method is described 

 for determining the genuineness and purity of seeds and feeding stuffs by 

 means of the precipitin reaction between the extracts of the material and the 

 serum of dogs immunized against the specific proteins of the pure material. 

 Typical results of the ;i]iplication of the method to the examination of certain 

 seeds are reported. 



Nitrogen balance during the manufacture of sugar. — precipitation of the 

 protein material of the sugar beet by sulphurous acid, bisulphites, and 

 hydrosulphites, E. Saillard {Comi}t. Rend. Acad. Sci. [Paris], 170 {1920), No. 

 2, pp. 129, ISO). — The author supplements his previous contribution on the com- 

 position of the different products obtained in the manufacture of sugar from 

 the sugar beet (E. S. R., 39, p. 417) by data on the nitrogen distribution in the 



