460 EXPERIMENT STATION RECORD. [Vol. 43 



extracted with 0.1 per cent HCl, and the extract concentrated in vacuo. 

 Tills extract, which gave a heavy precipitate with phosphotunj,'stir acid, an 

 insoluble precipitate with picric acid, and a negative biuret test, was found 

 to relieve the polyneuritic symptoms of pigeons. 



Tlie method of preparing an active material from dried yeast was as fol- 

 lows: The yeast, dried in a current of air at ordinary temperature, was 

 ground to a fine powder and extracted with 95 per cent methyl alcohol (2 cc. 

 of alcohol to each gram of yeast), 1 cc. of concentrated HCl being u.sed 

 for each liter of alcohol. The soluble part was filtered off by suction, and. 

 the residue again extracted as before, the two extract.s were combined, the 

 alcohol removed in vacuo at 35°, and the wax-like residue repeatedly extracted 

 with small volumes of ether and 0.1 iier cent HCl. This extract, when fur- 

 ther purified by the use of the Funk silver method and the mercuric sulphate 

 procedure, yielded an apparently crystalline substance which became inactive 

 on drying. The material gave a heavy precipitate with phosphotuiigstic acid 

 slightly soluble in excess, a negative biuret test, and no precipitate with picric 

 acid. It was highly active on polyneuritic pigeons. This method of isolation 

 eliminates from the active material purins, histidins, proteins, and albumoses. 



The antiscorbutic properties of concentrated fruit juices, A. Hakden and 

 R. RoBisoN {Biochem. Jour., IJ, {1920), No. 2, pp. i7/-l?7).— Samples of dried 

 orange juice, the preparation and properties of which have been previously 

 noted (E. S. R., 41, p. 470), were examined for antiscorbutic potency after 

 having been stored in a desiccator at room temperature for nearly two years. 

 Complete protection from scurvy was afforded guinea pigs weighing about 

 300 gm. by a daily ration of 0.5 gm. of the dried orange juice, equivalent 

 to about 4.5 cc. of the raw juice. 



Preliminary experiments are reported on large scale evaporation of the 

 orange juice to determine the practicability of preparing a highly active 

 product on a commercial scale. These experiments were discontinued after 

 the publication by Givens and McClugage of successful results along similar 

 lines (E. S. R., 41, p. 560). 



"The preparation on a commercial scale of such a dried orange juice ap- 

 pears to be quite practicable, and should prove of considerable value where 

 an antiscorbutic material is required in a highly concentrated and stable 

 form." 



The antineuritic and growth stimulating properties of orange juice, 

 A. H. Bytield, a. L. Danikls, and R. Loughlin (Amer. Jour. Diseases Children, 

 19 {1920), No. 5, pp. 349-358, figs. 5). — The antineuritic and growth-promoting 

 properties of orange juice were studied by a series of observations 

 carried out on babies under the same conditions as those reported in the study 

 by Daniels et al. of the role of the antineuritic vitamin in infant feeding (E. 

 S. R., 42, p. 256). 



By increasing the amount of orange juice from the 15 cc. usually given as 

 a daily antiscorbutic dose to 45 cc. a marked stimulation in growth resulted in 

 every case, the results being comparable with those obtained in the earlier 

 study with the use of wheat embryo extract as a source of the antineuritic 

 vitamin. Orange juice filtered after being shaken with kaolin caused no gain 

 in weight when fed in daily doses of 45 cc. When an equal quantity of un- 

 treated orange juice was given there was an immediate gain in weight, thus 

 showing that the growth-promoting substance was removed by the kaolin. 

 Similar results were obtained in experiments with rats. That the growth- 

 promoting factor was not identical with the antiscorbutic factor was proved 

 by the growth of rats receiving orange juice which had been Ijoiled for five 



