VETEBINAEY MEDICINE. 679 



"A number of the vaccinated animals may give a typical tuberculin reaction 

 following the vaccinations for a period of 20 months. These animals may or 

 may not show lesions of tuberculosis at autopsy. Vaccine administered to 

 animals already infected with tuberculosis is capable of retarding or holding 

 in check the progress of the disease. 



" The milk from innnunized cows when fed over a long period of time appears 

 to increase the resistance of calves and pigs. Vaccination of calves against 

 tuberculosis is of assistance in the eradication of tuberculosis from a herd if 

 done under the proper conditions. Until further knowledge is obtained in 

 regard to the destruction or outcome of the living tubercle bacilli constituting 

 the vaccine, no practical method for the immunization of milk-producing 

 animals under ordinary conditions can be advocated." 



Part 2 reviews the work on the production of immunity in cattle against 

 tuberculosis and the use of tuberculin. A complete bibliography of literature 

 cited in the review of the subject is appended. 



Ag'g'lutination test as a means of studying the presence of Bacterium 

 abortus in milk, L. H. Cooledge ( U. S. Dept. Agr., Jour. Ayr. Research, 5 (1916), 

 No. 19, pp. 871-875). — As a result of an investigation at the Michigan Experi- 

 ment Station, the author concludes that " a pure culture of B. abortus intro- 

 duced into the milk cistern of a cow's udder caused the appearance of agglu- 

 tinins in the milk. In every case in which B. abortus was found present in the 

 milk by animal inoculation the agglutinins for this organism were also found, 

 but this bacterium was not found in every case in which agglutinins were 

 demonstrated. The agglutination test is of value in studying the presence of 

 B. abortus in milk when it is desired to study a large number of samples. If 

 B, abortus is found to be pathogenic for human, this test may be of value 

 as another means of safeguarding certified and all unpasteurized milk." 



See also a previous note (E. S. R., 33, p. 774). 



The bactericidal action of methylene blue on abortion bacilli, F. B. Hadley 

 (Vet. Alumni Quart. [Ohio State Univ.], 2 (1915), No. 4, pp. 139-1^2) .—In this 

 paper the author reports the results of tests made of two different makes of 

 methylene blue. 



Three strains of the abortion bacillus were used in the experiments, con- 

 ducted in the veterinary laboratory at the University of Wisconsin, all of 

 which were satisfactorily cultivated after exposure to solutions of Merck's 

 medicinal methylene blue varying in strength from 1 : 1,000 to 1 : 4,000 for 73 

 hours. One particularly resistant strain was able to reproduce itself after 

 having been immersed in a 1 : 4,000 solution for 124 hours. Griibler's blue 

 appeared to be more toxic for the organisms. Accompanying tables record 

 the bactericidal effect of the chemical manufactured by the two different firms. 



These experiments indicate that under laboratory conditions methylene blue 

 does not destroy the abortion bacilli readily even in strong solutions, and should 

 not be considered a " sure cure " for the disease which they cause. It is 

 considered to be of little value as an external antiseptic. 



Brief history of the cattle tick fight in Louisiana to date, W. H. Dalrtmple 

 (La. Live Stock Sanit. Bd. Circ. 6 (1915), pp. 16). — This circular reviews the 

 history of the cattle tick control work carried on in Louisiana up to the present 

 time. 



Cell inclusions in hog cholera, L. R. Himmelbergee (Jour. Amer. Vet. Med. 

 Assoc, 48 (1916), No. 4, pp. 450-453, fig. i).— The author has systematically 

 examined smears from the conjunctival epithelium of a number of hogs and has 

 observed cell inclusions under various circumstances. He has never found the 

 inclusions in the epithelial cells after death and all cells did not contain them 

 even in a positive case, having, in fact, been found in but a small percentage of 



