680 EXPERIMENT STATION RECORD. 



the cells in a given microscopical field. These bodies were found in 95 per cent 

 of the cases examined during the period in which the body temperature was 

 beginning to rise, that is, in the first stages of the disease. In but a small 

 percentage of the cases were cell inclusions observed at the time the tempera- 

 ture was at its maximum (106 to 108° F.). Examination of sections taken 

 from a few cases at the time of death failed to show the presence of cell in- 

 clusions, and smears made from the conjunctiva of hogs after death gave 

 similar results. The actual significance of these bodies remains to be deter- 

 mined. 



A filterable org'anism isolated from the tissues of cholera hogs. D. J. Healy 

 and E. J. Gott {Jour. Infect. Diseases, IS (1916), yo. J, pp. 12.',-128, pi. 1).— 

 Continuing the work on hog cholera at the Kentucky Experiment Station, pre- 

 viously noted (E. S. R., 34, p. 582), the authors report that they have isolated an 

 organism from the mesenteric glands of hogs acutely ill with hog cholera. The 

 organism has been passed through an " F " Chamberland-Pasteur bougie and 

 successfully cultivated and stained. Complement fixation has been obtained 

 with the culture fluid in which the organism was grown. This fluid was not 

 able to fix complement previous to the growth of the organism in it. 



The value of virulent salt solution in the production of antihog-cholera 

 serum by the intravenous method, K. (Ikaham and L. H. Himmklberger {Jour. 

 Infect. Diseases, 18 {1916), No. 1, pp. 118-123). — In experimental work at the 

 Kentucky Experiment Station the use of salt virus in conjunction with blood 

 virus for the production of antihog-cholera serum has given satisfactory results. 

 In the work 25 co. per pound of body weight of a 0.9 per cent sterile salt .solu- 

 tion was injected into the i)eritoneal cavity of virus pigs five hours before kill- 

 ing, when the .solution was aseptically removed. In laboratories where all serum 

 is tested before being used in the field this method has proved a perfectly safe 

 and economical procedure. 



Sugg'estions relative to the prevention of hog' cholera, J. H. Kastle. R. 

 Graham, and II. K. Wright (Kcntuchu Sta. Circ. 10 {1915) pp. 70-80, figs. 

 4). — This bulletin contains practical .suggestions relative to the general care 

 and management of hogs ; precautions to be observed in purchasing new stock ; 

 the disposition of hog-cholera carcasses and proper disinfection ; the sale and 

 transportation of sick hogs; the prevention of hog cholera by means of hog- 

 cholera serum ; the construction of hog houses; the control of internal parasites; 

 and the use of some hog remedies. 



Effects of refrigeration upon the larvae of Trichinella spiralis. B. H. 

 Ransom (T. S. Dept. Agr., Jour. Agr. Research. .5 {1916). Xo. 18, pp. 819-854).— 

 This is a report of investigations conducted in continuation of those previously 

 noted (E. S. R., 30, p. 881). 



During the course of the work quantities of trichinous meat varying in 

 weight from a few grams to nearly 400 lbs. were frozen and kept for periods 

 varying from a few minutes to 57 days at various temperatures below the freez- 

 ing point of water. In most cases the period of refrigeration was between 5 

 and 20 days. About 33,0CX) trichinre were examined from artificially digesteil 

 frozen and unfrozen meat and over 500 test animals and control animals were 

 fed and examined. 



" A considerable proportion of the trichina? in meat exposed to a temperature 

 of about 15° F. for periods of 23 days or less survive and are quite lively after 

 thawing, but such meat frequently fails to infect test animals. This temi>era- 

 ture Is injurious to trichina^ but its effects are uncertain, and meat exiw.sed as 

 long as 23 days has proved to be infectious. Some of the trichime in meat ex- 

 posed to a temperature of about 10° for periods of 57 days or less generally sur- 

 vive, but the meat frequently fnils to infect test animals. A temperature of 10° 



