478 EXPEEIMENT STATIOIST RECORD. [Vol. 41 



School (Berlin)." The author is of tlie opinion that the complement deviation 

 method is superior to others for the serological detection of the disease. 



Note on the rapid production of serum against rinderpest and its utiliza- 

 tion in infected regions, M. CueaSson {Rec. MM. V6t., 95 {1919), No. 11, pp. 

 323-327). — Methods employed by tlie author for immunization against rinder- 

 pest are described in detail. 



Animals which have recently recovered from the disease are chosen for 

 hyperimmunization, and animals at different stages of the disease are used to 

 furnish the virulent blOod, In place of a single massive injection, two inocu- 

 lations of virulent blood are employed for immunization, the first coming from 

 an animal at the end of the first stage of the disease and the second, after an 

 interval of 3 or 4 days, from an animal at the second or third period. Each 

 inoculation of 3 or 4 liters is in itself sufficient to produce hyperimmunity. 



The blood for immunization is drawn into a sodium citrate solution in sterile 

 bottles. The immunizing blood is drawn four times, first on the tenth day 

 following the second virulent injection and then at intervals of four days. From 

 3 to 5 liters are taken each time, the blood being received in sterile bottles con- 

 taining 2 gm. of potassium oxalate and 0.25 gm. of phenol dissolved in 50 or 

 60 cc. of water. In place of oxalated blood, blood serum to which phenol is 

 added in the proportion of 0.25 gm. to a liter of the serum may be employed. Both 

 blood and serum are kept cool until used. 



As a preventive measure, the oxalated blood is injected in doses of 40 cc. for 

 adults and from 20 to 30 cc, for young animals. Virus in the form of virulent 

 blood, kept liquid by defribination or by the addition of sodium citrate, is in- 

 jected at the same time in 0.25 cc. doses. If immune serum is used in place of 

 the oxalated blood the doses should be diminished by one-half. 



As a curative measure the immune blood or serum is said to be of only limited 

 ""Value, acting with certainty only during the first stage of the disease. 



Swamp fever in Wyoming, — Economic importance, general characteristics, 

 and control, J, W. Scott {Wyoming Sta. Bui. 121 {1919), pp. 91-140, figs. 36).— 

 This bulletin was prepared for the purpose of bringing facts relating to the 

 nature of swamp fever, its economic importance, how it may be recognized, and 

 how controlled to the attention of ranchmen and farmers of the State. The 

 author calls attention to the fact that the experiments previously noted (E. S. 

 K., 39, p. 162) have conclusively demonstrated that the disease may be trans- 

 mitted by certain biting flies, a detailed report of which work will be presented 

 in a later paper. 



Diagnosis of tuberculosis by the complement deviation method, Chung 

 YiK Wa>'G and J., Crocket {Brit. Med. Jour., No. 3053 {1919), pp. 7-9).— An im- 

 provement in the method of jireparation of tubercle antigen for the diagnosis of 

 tuberculosis by complement deviation is reported which, together with certain 

 precautions in technique, is said to have rendered the method much more re- 

 liable as a diagnostic procedure. The nonspecific reaction which other ob- 

 servers have obtained is attributed by the authors to the presence of lipoids 

 in the antigens employed and to an insufficient heating of the tested serums. 

 To obviate these difficulties the tubercle bacilli employed as antigens in the 

 present study were freed from lipoids by 5 extractions alternately with chloro- 

 form and ether, and the serums were heated for at least two full hours at 

 55" <!!. The final technique employed and classification of results were similar 

 to those of the Wassermann test. 



The findings of the Cases investigated by this method were positive in 75 

 I)er'cent'of the 104 tuberculosis subjects tested, and none of the controls reacted 

 positively to the test after differentiation of Wassermann serums from tuber- 

 culosis by a chloroform differential method devised by the authors. It is, 



