576 EXPERIMENT STATION RECORD. [Vol.41 



Tile cokl-alcohol-soluble, hot-alcohol-soluble, and alcohol-insoluble fractions 

 of Witte peptone were unable to stimulate the production of precipitins or com- 

 plement binding antibodies when injected into rabbits. Anaphylaxis experi- 

 ments witli guinea pigs showed that these preparations had only slight power 

 of sensitizing to themselves, were not able to sensitize to beef serum, and did 

 not produce symptoms of anaphylactic intoxication in animals sensitized to 

 beef serum. Beef serum did not sensitize to products of Witte peptone nor 

 produce shock in animals sensitized to such products. 



The proteose preparations from egg white v.-ere obtained by hydrolysis of 

 the coagulated material, and fractional precipitation with ammonium sulphate, 

 lilxperiments with rabbits indicated that the 1/4, 1/3, 1/2, and 2/3 saturation 

 products possessed no power of stimulating the production of precipitins or 

 complement binding substances, and were unable to sensitize or intoxicate 

 guinea pigs either to themselves or to egg wliite. The 3/4 and 4/4 saturation 

 products showed definite antigenic power for both precipitins and complement 

 binding substances, and possessed slight sensitizing and intoxicating proper- 

 ties for guinea pigs. In all forms of antibody reaction the antigenic pro- 

 teoses were not specific, in that egg white could be used as antigen in place of 

 either fraction. 



Is hemoglobin antigenic? C. L. A. Schmidt and C. B. Bennett (Jour. Infect. 

 Diseases, 25 {1919), No, 3, pp. 207-212).— A brief review of the literature on the 

 properties of hemoglobin as a possible antigen is given, and experiments 

 initiated in the belief tliat tlie positive results obtained by some workers can 

 1)6 attributed to impurities in the preparation of hemoglobin are described and 

 summarized as follows: 



" Hemoglobin was prepared by three methods, one by crystallization from 

 ammonium sulphate . . . and the other two by precipitation as oxyhemoglobin 

 (with and without addition of alcohol) at low temperature and resolution by 

 reduction. Each of the three preparations was recrystallized a number of 

 times. 



" Rabbits were immunized with each of the above preparations and both 

 fixation and precipitin tests carried out. These were negative. In the sera 

 of these rabbits no sensitizer was found which in the presence of alexin caused 

 a hemolysis of dog cells. Agglutinins vrere likewise not produced. Hemoglobin 

 failed to sensitize guinea pigs for the anaphylaxis reaction. The conclusion 

 that hemoglobin is nonantigenic seems warranted. 



" Doses of hemoglobin very much larger than the amount of globin which 

 will cause toxic symptoms were injected both intravenously and intraperi- 

 toneally into guinea pigs, and toxic symptoms were not shown. Combination 

 of toxic globin with hematin as occurs in hemoglobin renders it nontoxic, but 

 does not, as in the instance when globin is combined with casein, give when 

 rabbits are immunized antibodies for itself." 



Prophylactic and curative value of serums and x^accines in veterinary 

 medicine, D. G. Izcaea (Bol Ayr. T6c. y Econ., 11 (1919), Nos. 126, pp. 516- 

 520; 127, pp. 595-604; 1^8, pp. 697-706). — This Is an extract from an address 

 delivered at the National Congress of Medicine (Spain). 



The first part deals with prophylaxis by normal virus, prophylaxis by at- 

 tenuated vii'us or vaccines, immunization with dead vaccines, virus, and sensi- 

 tized vaccines, immunization with serum alone (serum immunization) or with 

 serum associated with a virus or vaccine (serum vaccination), and sero- 

 therapy. In the second part the applicability of one or another of these 

 methods to the prophylaxis and treatment of various animal diseases is dis- 

 cussed. The diseases considei'ed include cowpox, foot-and-mouth disease, con- 

 tagious pleuropneumonia, rabies, tetanus, anthrax, glanders, gaseous gangrene, 



