782 EXPERIMENT STATION RECORD. [Vol.41 



through cheesecloth by means of a tincture press until 1,000 cc. of filtrate is 

 obtained. This is heated in streaming steam for 1 hour, filtered through 

 paper, and the volume made up to 1,000 cc. and titrated. The reaction is cor- 

 rected to approximately neutral with N sodium hydroxid, after wliich 1 per 

 cent of peptone and 0.5 per cent of sodium chlorid are added and the liquid is 

 lieated in streaming steam for 30 minutes. The final reaction is corrected to 

 pH 7.6. The medium is filtered through paper into tost tubes, 10 cc. per tube, 

 and sterilized in steam for one and one-half hours, or in an autoclave for 15 

 minutes at 15 lbs. pressure. 



Studies on anthelmintics, III— IV, M. C. Hall {Jour. Anier. Vet. Med. Assoc, 

 55 (1919), No. 6, pp. 652-659; 56 {1919), No. 1, pp. 59-70 ) .—Experiments in con- 

 tinuation of those previously noted (E. S. R., p. 480) indicate that chloroform 

 is safe, and is more efCective against hookworms in single dose than any other 

 nnthelmintic. 



In experiments with combinations of oil of chenopodium and chloi'oform, 

 " the best results in the removal of hookworms by a single-dose treatment 

 . . . were obtained by the use of oil of chenopodium in a dose approximating 

 0.1 mil per kilogram, as near as the dose can be approximated in 5 and 10 minim 

 soft, or soluble elastic, capsules, followed immediately by 30 mils of castor oil 

 containing chloroform at the rate of 0.2 mil per kilogram or with this amount 

 of chloroform given in hard gelatin capsules. . . . This same treatment 

 was 97 per cent effective against ascarids in tests on 15 dogs, of which 9 had 

 ascarids. The treatment was comparatively ineft'ective against whipworms, 

 which require repeated treatments, not single treatments. The treatment was 

 also ineffective against tapeworms, but neither of these drugs is dependable 

 against tapeworms." 



Further report on lymphangitis in cattle caused by acid-alcohol-fast or- 

 ganism, J. Traum {Jour. Amer. Vet. Med. Assoc, 55 {1919), No. 6, pp. 639-652, 

 figs. 5). — This is a further report of studies of the affection previously noted 

 (E. S. R., 36, p. 82). 



The investigations show the disease to resemble tuberculosis in the charac- 

 ter of the lesions and by the presence of acid-fast organisms in the lesions. 

 " It further resembles tuberculosis by the fact that a large percentage of cases 

 under disi-ussion give positive reactions to the intradermal tuberculin test; a 

 number of such reactors have been autopsied . . . and in none of the cases 

 did we find tuberculosis ; in fact, in some of the reactors tuberculosis could be 

 definitely eliminated. 



" That it is not tuberculosis is satisfactorily demonstrated by the failure to 

 produce a single case of tuberculosis in the large number of guinea pigs Inocu- 

 lated with materials from the above-described and other cases. The organism 

 isolated from lesions and described here as the probable causative agent re- 

 sembles tubercle bacilli to some extent, differing, however, sufficiently in the 

 character and speed of growth and microscopic appearance of smears from the 

 cultures to easily differentiate it from tubercle bacilli." 



Iris poisoning of calves, E. A. Bruce (Jour. Amer. Vet. Med. Assoc, 50 

 {1919), No. 1, pp. 72-74). — The author records the death of several calves from 

 3 to 6 months of age from having fed on the leaves of a blue-flowering bulbous 

 variety of iris grown under cultivation at Abbottsford, B. C. 



Don't feed foxtail hay to lambing ewes, C. E. Fleming and N. P. Peterson 

 {Nevada Sta. Bui. 97 {1919), pp. 18. fiffs. 12).— This is a report of studies made 

 of the injurious effects of feeding ewes during the lambing season on hay 

 containing quantities of squirrel tail grass {Hordeum jubutum), also known as 

 tickle or foxtail grass and wild barley, a recent account of which grass by 

 Pammel in Iowa has been noted (E. S. R., 39, p. 842.) 



