80 EXPEKIMENT STATION RECORD. 



T. simice multiplies in the iutestines and in the labial cavity of the proboscis 

 of Glossina morsitans. Here only developmental forms are found, never in- 

 fective forms. The T. simiw growing in the intestines of the ' fly ' has no spe- 

 cific characters by which it can be distinguished from other species of pathogenic 

 trypanosomes found in tsetse flies. The final stage of the development takes 

 place in the hypopharynx, wherein the infective form of the parasite, similar in 

 shape to the trypanosome found in the blood of infected animals, is produced. 

 The flies do not become infective until about 20 days after their first infected 

 feed. 



Trypanosome diseases of domestic animals in ITyasaland. — II, Trypano- 

 soma caprse, D. Beuce et al. {Proc. Roy. Soc. [London], Ser. B, 86 (1913), 

 Ko. B 587, pp. 278-284, pi. 1, fig. i).— " T. caprw belongs to the same group as 

 T. vivax and T. uniforme, and affects the same animals, cattle, goats, and sheep. 

 Monkeys, dogs, and the smaller laboratory animals are immune. The carrier 

 is Glossina morsitans. The reservoir of the virus is the wild game living in the 

 * fly country.' " 



The trypanosomes found in the blood of wild animals living in the sleep- 

 ing- sickness area, Nyasaland, D. Bruce et al. (Proc. Roy. Soc. [London], 

 Ser. B, 86 (1913), No. B 587, pp. 269-277). — "Thirty-one and seven-tenths per 

 cent of the wild game in the * fly country ' below Kasu Hill harbor pathogenic 

 trypanosomes. The species of trypanosomes found are Trypanosoma 'brucei vel 

 rJiodesiense 7.8 per cent, T. pecorum 14.4, T. simice 1.7, T. caprw 11.1, and T. 

 ingens 1.7. It is self-evident that these wild animals should not be allowed to 

 live in ' fly country,' where they constitute a standing danger to the native in- 

 habitants and the domestic animals. . . . Active measures should be taken 

 for their early and complete blotting out. . . . 



" No pathogenic trypanosomes have up to the present been found by the com- 

 mission in the blood of animals living in fly-free areas." 



Morphology of various strains of the trypanosome causing disease in 

 man in Nyasaland, D. Bruce et al. {Proc. Roy. Soc. [Londoti], Ser. B, 86 

 {1913), Nos. B 589, pp. 394-^07, figs. 7; pp. 408-421, figs. 7; B 592, pp. 26-35, pis. 

 S. figs. 2). — The authors conclude that the 5 wild game strains resemble each 

 other closely, and all belong to the same species of trypanosome {Trypanosoma 

 rliodesiensc (Stephens and Fantham).) The human strain differs to some 

 extent, but also belongs to the same species. " There is some reason for the 

 belief that T. rhodesiense and T. hriicei (Plimmer and Bradford) are one and 

 the same species." 



" The trypanosome of the Mzimba strain is the snme species as that occurring 

 in the wild game inhabiting the Proclaimed Area, Nyasaland. It has already 

 been concluded that this species is Trypanosoma brucei vel rJiodesiense. Hence 

 it would appear that wild Glossina morsitans occurring in a district 100 miles 

 north of the Proclaimed Area are infected with the trypanosome which causes 

 the human trypanosome disease of Nyasaland." 



Studies on the biochemistry and chemotherapy of tuberculosis, IV, V, VI 

 (Jour. Infect. Diseases, 12 {1913), Nos. 1, pp. 68-92; 2, pp. 249-275) .—These 

 parts continue previous work (E. S. R., 29, p. 177). 



IV. Preliminary report of experiments in the vital staining of tubercles, 

 Lydia M. DeWitt (pp. 68-92). — "Among the dyes so far tested, trypan blue, 

 trypan red, isamin blue, pyrrhol blue, Ehrlich's rectified methylene blue, medici- 

 nally pure methylene blue, methylene blue of the U. S. Pharmacopoeia, new 

 methylene blue N, new methylene blue GG, and to some extent neutral red and 

 pyronin have been found to penetrate tubercles in guinea pigs. Basic fuchsin, 

 crystal violet, and the other new methylene blues are now being tested. . , . 



