484 EXPERIMENT STATION RECORD. 



the impression that the power of tolerance might eventually be lost and that 

 the elimination would then be complete. Experiments were accordingly made 

 to determine the period of retention of the bacilli used for the test inoculation. 

 The bacilli were subcultivated for a long period of time on glycerinized ox bile. 

 Eight heifers which did not react toward the tuberculin test were used in the 

 experiments. " Seven of them were killed 1, 2, 3, 4, 8, 12, and 18 months, re- 

 spectively, after the test inoculation. At the post-mortem of each the bronchial 

 glands were removed, triturated in their entirety, and injected subcutaneously 

 into 12 guinea pigs. The latter were killed 2 months later. It was found that 

 while none of the vaccinated heifers showed any lesions of tuberculosis at the 

 post-mortem, every one of the guinea pigs inoculated was tuberculous. It was 

 thus proved that a vaccinated animal might carry the vii'ulent bacilli used for 

 the test inoculation in its glands for 18 mouths aftei- the test inoculation with- 

 out their producing any lesions, although their continued virulence could be 

 proved. 



" The eighth heifer was tested with tuberculin 18 months after the test 

 inoculation and did not react, although she no doubt harbored virulent bacilli 

 in her glands as did the 7 that were killed. A second inoculation of 3 mg. 

 of virulent bovine bacilli was given, but there was no elevation of tempera- 

 ture, and 3 months later the animal had every appearance of being in per- 

 fect health. The authors conclude that their experiments indicate that when 

 animals are vaccinated with attenuated bacilli of the bovine type and are 

 then subjected to a test inoculation, they do not develop a^iy evidence of tuber- 

 culosis, but they retain virulent bacilli in their glands for periods extending 

 into months. A number of the bacilli are passed out gradually with the 

 excreta, as proved by guinea pig inoculations." 



The vaccination of bovines against tuberculosis with bacilli of bovine origin 

 possesses great advantages, but it is necessary to have an attenuated strain 

 of which considerable doses can be given to bovines with ease, and which are 

 not capable of producing lesions. The strain possessed by the authors is sup- 

 posed to fulfill these requirements. It is avirulent for the ox, monkey, and 

 guinea pigs, and confers a lasting resistance on bovines when given intra- 

 venously, but the duration of the immunity has not been established. 



The hog cholera question, J. Pekae (Berlin. Tierdrzth Wchnschr., 29 (1913), 

 No. 26, pp. 467-^69). — A polemic in regard to priority and a restatement of the 

 facts about septic endometritis and abortion present in hog cholera. The 

 author believes that classical hog cholera, swine plogue, and septicemia can 

 be produced in pigs of all ages by simply feeding them in uterine secretion 

 obtained from hogs affected with hog cholera, swine plague, etc. The condi- 

 tion produced is accompanied by an endometritis. Methods for treating the 

 above are included. 



What the agricultural experiment station hog serum laboratory is doing 

 for California hog raisers, C. M. Haring (Univ. Gal. Jour. Agr., 1 (1913), No. 

 1, pp. 8-13, figs. 3). — This paper gives an account of the work being carried 

 on in the preparation and distribution of antihog cholera serum in California. 



About pyobacillosis in pigs and serum treatment for the same, O. Sten- 

 STROM (Svensk Vet. Tidskr., 11 (1912), No. 9, pp. 293-297; abs. in Berlin. 

 Tierdrztl. Wchnschr., 29 (1913), No. 26, p. 471).— In an epizootic in which a 

 great many pigs died five shoats on autopsy were found to have a pleuro- 

 pneumonia, and from parts of the lungs, etc.. the Bacillus pyogenes could be 

 isolated. Of 60 pigs, 2 to 4 weeks old, treated with 2.5 cc. of B. pyogenes 

 serum (Jensen), none died, while 2 animals kept as controls succumbed. The 

 author concludes that the serum when used in large doses (15 cc.) has cura- 

 tive properties. 



