VETERIXAEY MEDICINE. 481 



Immunizing against glanders, De Blieck aud Bubbermann (Ahs. in Berlin. 

 Tierarzll. Wchnschr., 29 (1913), No. 26, pp. JpO, ^7i).— Farase yielded fair 

 results wblle those obtained with heated cultures of glanders bacilli (malleus- 

 vaccin) were entirely unsatisfactory. With the latter toxin the disease was in- 

 creased in extent, one of the two horses treated dying six weeks after the in- 

 jection aud the other being destroyed on account of being very cachectic. A 

 control animal died eight weeks postinfection. Five horses were treated with 

 farase, four with the powdered preparation, and one with the liquid product. 

 On autopsy it was found that none of the animals absolutely withstood the 

 infection, but with only one exception all animals showed a partial immunity. 



The canine piroplasmoses of Europe and Africa, A. Laveran and Nattan- 

 Larbier {Ann. Inst. Pasteur, 21 {1913), No. 9, pp. 701-717, figs. 5).— Of seven 

 dogs which were inoculated with the African virus after having been immunized 

 with the French virus six succumbed to piroplasmosis. Xwo dogs inoculated 

 with the French virus after having been immunized with the African virus suf- 

 fered a light infection which terminated in recovery. The authors conclude 

 that the African piroplasm, if not a separate species, is at least a variety dis- 

 tinct from the piroplasm occurring in dogs in France. 



The culture of Babesia (Piroplasma) canis in vitro, J. G. Thomson and 

 H. B. Fantham {Ann. Trap. Med. and Par., 7 {1913), No. If, pp. 621-632, pi. 1, 

 figs. 5). — "We have succeeded in cultivating B. canis in two out of four 

 attempts, following the method of Bass, using blood and glucose, and incubating 

 at 37° C. In one of these cultures, starting with heart blood containing cor- 

 puscles infected with one, two, or, exceptionally, four piroplasmata, we suc- 

 ceeded in obtaining a maximum of 32 merozoites in a corpuscle. 



" Various types of Babesia were seen in these cultures, namely, pyriform, 

 ameboid, rounded, and oval parasites. Division of rounded forms was ob- 

 served, following the method of gemmation with chromatinlc forking. There 

 was evidence, in stained specimens, of direct binary fission. Hemolysis occurred 

 in all the culture tubes. A puppy was successfully inoculated from a 41 hours' 

 culture and succumbed to piroplasmosis on the fifth day. B. canis is not so 

 easily cultivated by Bass's method as the malarial parasites of man." 



On the cultivation of the malarial parasites and of piroplasms (Piroplasma 

 canis) in vitro, H. Ziemann {Arch. Schiffs u. Tropen Hyg., 17 {1913), No. 11, 

 pp. 361-391, pis. 2, figs. 3). — The author reports upon his successful cultivation 

 of P. canis, and gives a bibliography of 21 titles. 



On the multiplication of Piroplasma canis in vitro, Knuth and Richters 

 {Berlin. Tierdrztl. Wchnschr., 29 {1913), No. 12, pp. 211, 212; ahs. in Rev. G6n. 

 MM. V6t., 21 {1913), No. 248, p. 462).— The authors report having succeeded In 

 cultivating P. canis and here describe the method employed, which is a modifica- 

 tion of the Bass method of cultivating the malarial organisms. The best results 

 were obtained with a mixture of equal parts of the deflbriuated blood of a 

 mildly infected dog and of a 2 per cent glucose solution, kept at room temper- 

 ature. 



About the formation of an acute anaphylaxis from acid-fast bacteria and 

 from the neutral fat of the tubercle bacillus, E. Leschke {Ztschr. Inimuni- 

 tdtsf. u. Expt. Ther., I, Orig., 16 {1913), No. 5-6, pp. 619-626).— The results 

 show that by treating acid-fast pathogenic and nonpathogenic organisms with 

 normal serum, it is possible to produce an acute lethal poison. The toxin can 

 also be produced from neutral fats and from the fat of the tubercle bacillus 

 (tuberculonastin) by treatment with normal serum. 



Notes on the biolog'y of the tubercle bacillus, T. Smith {Jour. Med. Re- 

 search, 28 {1913), No. 1, pp. 91-110). — "Tubercle bacilli of both human and 



