580 EXPEKIMENT STATION RECORD. 



nlly. If healthy rabbits are injected with tuberculin and receive a second dose 

 within 17 hours the second dose lilve the first is without any injurious effects. 



The simultaneous application of tuberculin by the hypodermic, ophthal- 

 mic, and cutaneous methods, IT. Selan (Clin. Vet. [Milan], Scz. Prat., 31 

 (1908), A'O. 12, pp. 195-198). — A tuberculin test was made on 22 cattle, to which 

 the three methods of using tuberculin were applied simultaneously. The hypo- 

 dermic method gave reliable negative or positive results according as the ani- 

 mal was free from or affected with tuberculosis, the cutaneous and ophthalmic 

 methods, on the other hand, being negative in all cases. 



Ophthalmo and cuti-reaction in bovine tuberculosis, Garth, Kranich, 

 and (JRUNERT (Deut. Ticrurztl. M^chnschr., 16 (1908), No. U,, pp. 197-202).— In 

 testing the ophthalmo-reaction and cuti-reaction to tuberculin the authors found 

 that no reaction could be obtained unless a strong tuberculin was used. It also 

 a])peared to be necessary to proceed with unusual caution, otherwise the ex- 

 periment was in all cases doomed to failure. 



The cutaneous and conjunctival reaction to tuberculin, H. Wildbolz 

 (Berlin. Klin. Wchnschr., JiB (1908), No. 11, pp. 5.'io, 5^6). — The experiments 

 reported in this paper were made on rabbits, which the author believes to be 

 better adapted for experimental tests with tuberculosis than are guinea pigs. 

 The conjunctival reaction was in all cases of shorter duration than the cuta- 

 neous reaction. The former reached its maximum after about S to 10 hours 

 and disappeared after 48 hours. The cutaneous reaction reached its highest 

 I)oint on the second or third day and ]»ersisted for 4 or f) days. 



The specific value of conjunctival reaction to tuberculin, F. Arloing 

 (Compt. Rend. Soc. Biol. [Paris], 6', (W08), No. /J, /)/). 722-72-'/ ) .—In the 

 experiments /carried on by the author it appeared that the conjunctival reaction 

 to tuberculin is not specific. When tuberculin was instilled into the eye 6 

 hours after an injection of diphtheria toxin a pronounced general reaction took 

 place with all of the usual symptoms observed in a positive tuberculin test. 



The increased susceptibility to tuberculosis as a result of tuberculin in- 

 jection, A. Slatineano and D. Danielopol (Compt. Rend. Soc. Biol. [Paris], 

 61/ (1908), No. 9, pp. .'il8, 'il9). — In experiments with guinea pigs it was found 

 that no increased susceptibility to tuberculosis was apparent after the injection 

 of tuberculin until 4 days had elapsed. On the fifth day a considerably 

 increased susceptibility was apparent, the guinea pigs developing very acute 

 and generalized cases of tuberculosis when inoculated with virulent tubercle 

 bacilli 5 days after the tuberculin injection. 



The passage of tubercle bacilli into the milk, A. Coquot (Bui. Soc. Cent. 

 M(-d. Yet., 85 (1908), No. 6, pp. l-',6-152, fig. 7).— Tubercle bacilli were intro- 

 duced directly into the mammary artery of a cow and this cow was later slaugh- 

 tered and examined with reference to the possible location of the tubercle bacilli. 

 Soon after inoculation the milk was examined at frequent intervals to determine 

 whether tubercle bacilli had passed into it. The temperature of the animal was 

 somewhat elevated by the inoculation but no inflammation occurred in the 

 udder. Milk drawn from the udder under careful aseptic precautious was used 

 in inoculating guinea pigs but proved in every instance not to be tuberculous. 



The milk of reacting' but not clinical cases of tuberculosis in cows, K. 

 OsTERTAG (Ztschr. Fleisch u. Milchhyg., 18 (1908), No. 7, pp. 205-211).— The 

 results obtained by various investigators in studying the milk of cows which 

 react to tuberculin but do not show clinical signs of tuberculosis are critically 

 reviewed by the author and a brief account is given of experiments recently 

 undertaken by him along this line. The milk of 18 reacting cows was inocu- 

 lated into guinea pigs without producing any result and similar negative results 



