682 EXPERIMENT STATION EECORD. 



(1908), No. 6, pp. 298-301; ahs. in Bui. Inst. Pasteur, 6 (1908), No. 9, p. JiOt).— 

 Experiments were conclucted in which tubercle bacilli of bovine origin were 

 introduced directly into the stomachs of young pigs. Examination of one 

 killed 22 hours later showed the presence of the bacilli in the blood, in the 

 lungs, and in the mesenteric glands. In a second which died on the fourth day 

 following the infection the bacilli were found in the liver, lungs, and kidneys. 

 In a third which lived 3 weeks after the infection bacilli were found in the 

 blood, lungs, and mesenteric glands. It appears that tubercle bacilli pass within 

 a comparatively few hours into the blood and lungs and that they can remain 

 for a considerable period in a latent state without the appearance of lesions. 



The penetration of the intestinal wall by tubercle bacilli, M. Herman (Bnl. 

 Acad. Roy. Med. Belg., //. ser., 21 (1901), No. 8-9, pp. 5.',o-.556 ; abs. in Bui. Inst. 

 Pasteur, 6 (1908), No. 9, p. 406). — In experiments made, negative results w^ere 

 obtained from a monkey. Positive results were obtained in a goat of 4 years, 

 in all of 4 adult guinea pigs used, in 3 of 4 young guinea pigs, and in one of 5 

 rabbits. 



The simultaneous inoculation subcutaneously of a pathogenic bacillus 

 (Sirault's bacillus of meat poisoning) appears to favor the passage of the bacilli 

 through the mucous membrane by lowering the vitality. The irritation of the 

 intestine through the ingestion of the same bacillus appears to have rather 

 prevented the passage. 



Split products of the tubercle bacillus and their effects upon animals, 

 V. C. Vaughan and S. M. Wheeler (Abs. in Med. Rec. [N. Y.],71 (1901), No. 25, 

 p. 1051). — The tubercle cell was split up into two portions — cell poison and cell 

 residue. The cultui*al medium was concentrated and precipitated with five 

 times its volume of absolute alcohol and this precipitate then broken up into 

 two portions, the precipitate poison and the precipitate residue. The portion 

 of the cultural medium left after the removal of the alcoholic precipitate was 

 designated as the final filtrate. 



Single doses of the cellular substance were injected into the abdominal cavities 

 of 24 guinea pigs in doses varying from 5 to 200 mg. In no case was death 

 caused directly by the injection, and it gave no immunity to a subsequent in- 

 oculation with a virulent bacillus. 



The toxic effect of the cell poison on animals was shown to be through the 

 respiratory center. When given in sufficient quantities it killed within an hour 

 both healthy and tubercular animals. There was no evidence that it elaborated 

 any antitoxin, and it was harmful with nothing to recommend it. What was 

 true of the cell poison was equally true of the precipitate poison and the final 

 filtrate. The effects of the cell i-esidue on animals were practically nil. The 

 precipitate residue had no recognizable ill effects ui)on healthy animals but 

 had some specific effect upon tuberculous animals. 



Vaccination against tuberculosis in cattle with bovovaccine, H. L. Russell 

 and C. Hoffmann ( Wisconsin Hta. Bill. 165, pp. 3-13). — Experiments were made 

 to determine the extent to which bovovaccine immunizes cattle. Thirty-four 

 head of young stock all under 6 months of age that had passed the tuberculin 

 test, though taken from an infected herd were injected May 9, 1906, with 

 bovovaccine and isolated until after a second injection 3 months later. In order 

 to test the infectiousness of the barn originally occupied, 10 nonreacting calves 

 not vaccinated were placed with the vaccinated stock on July 6 to serve as a 

 control. After the second vaccination all experimental animals were pastured 

 with the infected stock and from the beginning of November all animals were 

 housed iu the infected baru, Tuberculin tests were made in 3, 5, 8, 14, and 17 



