404 p:xperiment station record. 



iionmotile and the motile race!^ of lio<j:-cholera bacillus and also Fxirilhin ideroides 

 show a close aflinity in the presence of ininmne agj^lutinins. The action of the serum 

 of rabbits and guinea pigs inununized with tlie nonmotile bacillus is quite different 

 toward this bacillus from the action of serum produced by motile bacilli. The clump- 

 ing appears later and in the form of a precipitate of grainilcs. Agglutination appears 

 also only after a higlier degree of immunization. 



During these studies it was found that the agglutinin which acts upon the flagella 

 is <iuite distinct from that which acts upon the body of bacteria. The agglutinin 

 wliich is active toward the bodies of nonmotile hog-cholera bacilli was found to be 

 identical with that which acts upon the bodies of tlie motile race of the same species. 

 A much higher degree of imnmnity is required in the experimental animal in order 

 to obtain the agglutinin which operates upon the body of the bacilli, while flagellar 

 agglutinins are quite easily demonstrated in the immune sera. 



The ag-glutination affinities of related bacteria parasitic in different hosts, 

 T. Smith and A. L. Reagh (Joiu: Med. Research, 9 {1903), No. -3, pp. 270-300).— \n 

 elaborate study was made of bacilli belonging to the hog cholera group of both motile 

 and nonmotile forms. The organisms studied included typhoid bacillus, coii bacil- 

 lus, BariUa.'< ideroides, hog-cholera bacillus, etc. During these investigations it was 

 found that there are certain agglutination relationships between pathogenic bacilli 

 which cause fermentation of dextrose. The agglutinative characters appear to be 

 moditied when the organism becomes parasitic upon different hosts. It was found 

 possible to predict agglutinative affinities from close pathogenic relationships. Guinea 

 pigs immunized with the same cultures in the same manner yielded sera with iden- 

 tical agglutinins. 



The appearance of agglutinins after cutaneous infection, W. Hoffmann 

 (HH'j. liundsclniti, 13 (1903), Xn. 3, jiji. 114-121). — The author conducted experiments 

 for the purjiose of determining whether it was possible to produce agglutinins in the 

 blood serum as a result of mere cutaneous inoculation. The exj^eriments demon- 

 strated that agglutinins are produced in rabbits after cutaneous inoculation with 

 typhoid bacillus. The agglutinative standard is not as high as that which is secured 

 by intravenous injections. 



The agglutinating- properties of bile, A. Cantaxi {Centbl. Bakt. u. Par., 1. Alt., 

 S3 {1903), No. 9, Orig., pp. 730-740). — The author made a large number of experi- 

 ments for the purpose of determining the possible agglutinating power of the bile 

 from normal and affected animals. It was found during these experiments that the 

 bile of normal rabbits, guinea pigs, and cattle possesses no agglutinating properties 

 toward the majority of bacteria, while the bile of dogs exhibits slight agglutinating 

 action. A pronounced power was observed in the bile of animals which had been 

 imnumized against certain bacterial organisms, such as the tyjihus and coli bacilli. 



The bactericidal action of the bone marrow and the etiology of osteomye- 

 litis, A. He.n'cke {C'enihl. Bakt. u. Par., 1. Aht.,33 {1903), No. 9, Orig., pp. 697-701).— 

 During the author's experiments, which were made chiefly on rabbits, it was found 

 that the bone marrow possesses quite striking bactericidal i^roperties. It recovers 

 from infection more quickly and completely than the internal organs. Staphylococcus 

 aureus introduced in pure cultures into the animal organism, whether into the blood 

 or into the bone marrow, failed to produce an infectious osteomyelitis. In 4 spon- 

 taneous cases of this disease investigated by the author, in which there was no com- 

 munication with the outside world, and in 1 case in which the communication 

 existed, small bacterial rods were found and were isolated in pure cultures. These 

 cultures were found to be capable of pro<lucing characteristic osteomyelitis when 

 inoculated intravenously. 



Determination of the bactericidal and antiseptic action of Bacillol, L. Hau- 

 MAX {Ing. Agr. Gemhloux, 13 {1903), No. 12, pp. 541-546). — A number of experiments 

 wei"e made for the purpose of determining the antiseptic jiower of Bacillol upon the 



