220 EXPERIMENT STATION RECORD. 



be made along these lines, and also concerning the organic acids normally present in 

 pure maple products. 



On the determination of fat in milk poor in fat, T. S. Thomsen {Landw. Vers. 

 Stat, 62 {1905), No. 4-5, }>}>. 887-399).— Experiments were made with buttermilk, 

 skim milk, and various mixtures and dilutions of these with water. The Gottlieb 

 method gave higher and, according to the author, more reliable results than the 

 extraction method. The results of fat determinations on buttermilk and skim milk 

 before and after peptonizing led to the conclusion that the impurities in the fat 

 obtained by the Gottlieb method are very slight. 



A study of the Htibl, Hanus, and Wijs methods of iodin absorption, H. L. 

 Wilson- ( Pennsylvania Sta. Rpt. 1904, pp. 146-150).— The work here reported was done 

 in cooperation with the associate referee on fats and oils of the Association of Official 

 Agricultural Chemists, the results of which have already been noted in the report of 

 the referee (E. S. R., 15, p. 438). 



Some notes concerning- Halphen's test for cotton-seed oil, E. Fulmer ( Wash- 

 ington Sta. Bui. 67, pp. 3-10).— Noted from another publication (E. S. R., 14, p. 836). 



Reaction of lard from cotton-seed meal fed hogs with Halphen's reagent, 

 E. Fulmer ( Washington Sta. Bui. 67, pp. 11-27).— Noted from another publication 

 (E. S. R., 16, p. 226). 



The precipitation limits with ammonium sulphate of some vegetable pro- 

 teins, II, T. B. Osborne and I. F. Harris (Amer. Jour. Physiol., 13 (1905), No. 5, 

 pp. 436-447). — The present paper is a continuation of work previously noted (E. 

 S. R,, 15, p. 222). The principal conclusions drawn from the investigations are as 

 follows: 



" Hofmeister's method of fractional precipitation of proteins from their solutions 

 by ammonium sulphate affords, in most cases, a valuable and ready means for 

 the separation of such substances when associated in the same solution. 



" The precipitation limits are not characteristic for each individual protein sub- 

 stance, as is commonly assumed, but appear to depend on the conditions existing in 

 the solution at the time of precipitation. . . . 



"Vegetable globulins can not be distinguished from vegetable albumins by means 

 of their precipitation limits with ammonium sulphate, since many of these globulins 

 are not precipitated until the concentration in ammonium sulphate is raised well 

 above one-half saturation, while leucosin, the best characterized albumin of vege- 

 table origin now T known, is almost completely precipitated at one-half saturation." 



The glycocoll content of different proteids, F. Dubrowin (lnaug. Diss., St. 

 Petersburg, 1902; abs. in Physiol. Puss.', 3 (1904), Nos. 48-60, p. 184).— Cleavage was 

 induced in a large number of proteids by treatment with sulphuric acid. No glyco- 

 coll group was found in the vegetable proteids examined. On the other hand, the 

 proteids of connective and skeletal tissue gave large quantities of glycocoll. Judged 

 by the glycocoll content of their cleavage products collagen, ossein, chondin, and 

 elastin are closely related. The proteid of the crystalline lens of the eye was found 

 to contain 4.5 per cent ty rosin and 0.7 per cent glycocoll. No glycocoll was found 

 in the cleavage products of myosin. 



Determination of hippuric acid in urine, R. E. Stallings (Pennsylvania Sta. 

 Rpt. 1904, pp. 151-154). — Using a modification of Konig's method, hippuric acid, uric 

 acid, and benzoic acid were determined in samples of urine of a steer, and also to test 

 the method in an artificial mixture of these acids to which a small amount of sodium 

 hydroxid had been added. In the case of the artificial mixture, the results obtained 

 for hippuric and uric acids were too low. The greatest discrepancy w T as observed with 

 the uric acid, while the benzoic acid was too high. In the case of urine, fairly con- 

 cordant results were obtained. 



