922 ON THE EXAMINATION OF WATER FROM THE SYDNEY SUPPLY, 



Bacillus E. 



Microscopical Characters. About -006 mm. long and '0018 mm. 

 broad cylindrical rods ; occur in filaments, consisting of a great 

 many individual rods, rounded at their extremities ; no spon- 

 taneous movement. 



On gelatine-plates. This form was only now and then met with 

 in a few colonies. At the beginning they consist of but a few 

 short threads crossing one another in various directions. They 

 multiply pretty quickly, and after about three days present greyish, 

 cloudy masses, having a central, darker, rounded part, where the 

 gelatine is liquefied in a watch-glass-like manner, and from which 

 delicate and multifariously ramified, and more or less elongated 

 threads or filaments radiate, spreading themselves at a good distance 

 over the surface of the gelatine. Besides that, I once saw a colony 

 propagating in a similar manner at the bottom of the gelatine on 

 the glass-plate. This micro-organism, therefore, is aerobic as well 

 as anaerobic. 



In nutrient gelatine in a test-tube it grows, like Bacillus 

 C, in the shape of an inverted cone, which, however, in this 

 case approaches somewhat that of a cylinder. We have here 

 a remarkably beautiful growth of extremely delicate, cloudy, and 

 wool-fibre-like appearance ; it is scarcely visible in reflected light, 

 and reminds one vividly of a test-tube pure-cultivation of the 

 bacillus of mice-septicaemia (Koch). It multiplies, in the 

 interior of the gelatine, far more readily than does Bacillus C (see 

 above). At the surface, where liquefaction of the gelatine 

 begins, the micro-organism offers at first pretty much the same 

 aspect as in its cultivation on plates ; later on, with the advancing 

 liquefaction of the gelatine, occurring from above downwards, it 

 represents here a greyish-white film, covered by a liquid mass. 



On a sloping surface of nutrient agar-agar it develops a 

 luxuriant superficial vegetation of a grey-white colour. In the 

 middle it is denser and more compact, being here folded up and 

 provided with small prominences. At the edges it is thinner and 

 has a lint-like appearance, being composed here of densely packed, 



