BY OSCAR KATZ, PH.D., M.A. 927 



and here it commences to liquefy the latter in a funnel- or watch- 

 glass-like manner, besides spreading itself beyond the margin of 

 this excavation in the shape of a very thin, fragmentary, greyish- 

 white film over the surface of the gelatine. Afterwards the 

 growth in the gelatine presents an inverted conical bag filled with 

 yellowish, densely packed flocky masses of the micro-organism. 

 The liquefaction advances from above downwards, till, in course of 

 time, there is seen in the test-tube one liquid mass, in which light, 

 filamentous, fibrine-like masses (belonging to the micro-organism) 

 are suspended, and at the bottom a noticeable deposit of yellowish 

 colour. 



On a sloping surface of a 1 p.c. nutrient agar-agar in a test- 

 tube the organism multiplies readily at ordinary temperatures, and, 

 after some dajs' standing, the cultivation presents a greyish, flat, 

 superficial growth which appears wrinkled or folded. This is 

 principally marked along the streak of inoculation, where the 

 vegetation, being also more luxuriant, looks as if covered with 

 short yellowish-white threads, interwoven with each other. 



On the cut surface of a sterilised potato the Streptococcus like- 

 wise readily propagates. (PI. XII, fig. 4.) After some time of 

 incubation at ordinary temperatures, it developes to considerable 

 masses of a peculiar yellowish-white tint and creamy consistency- 

 These masses do not extend far over the nutrient surface. They 

 resemble, following the track of the inoculating platinum wire, an 

 elongated chain of mountains, the edges of which are characteris- 

 tically emarginated, as if lined all along their contours with small, 

 bead-like prominences. All over the surface of these plateaux, 

 and quite close to one another, very shallow furrows are visible 

 which run down to the borders of the masses ; (conf. PI. XII, fig. 4, 

 where this appearance is roughly indicated.) 



I may well note here that with the propagation of the Streptococcus 

 a peculiarly sour but not very strong smell was associated, which 

 was especially noticeable in the pure culture of the bacterium on a 

 potato in a small glass capsule. 



