34 the method of section-cutting with improvements, 

 Staining the Sections. 



I have alluded above to the difficulties connected with staining 

 in toto, and will now dwell on the staining of the sections. They 

 are of course made just in the same way as described above. The 

 staining process is omitted. The specimen is placed in the 

 weakest alcohol, as soon as the hardening re-agent has been 

 washed out. The further process as above until the sections on 

 the slide are taken out of the turpentine bath. Haswell uses 

 colours mixed with turpentine or similar oils for staining his 

 sections an equally simple as commendable method. The slides 

 are removed from the turpentine bath and placed in a solution of 

 the desired colour in turpentine or any oil. Any transparent 

 oil-colour can be used for the purpose. Carmine yields also here 

 very good results. 



The ordinary staining-reagents can also be used, but this 

 requires a complicated and to the sections injurious process of 

 washing. The turpentine must be washed out with absolute 

 alcohol. This must be replaced by spirits of wine and finally 

 water. Then the sections are stained, after which they must be 

 washed, and treated with increasing strengths of alcohol as before, 

 finally they must be covered with clove oil and then mounted in 

 resin as above. If the staining is performed with an oil-colour, 

 the sections are immersed for a few minutes in the colouring 

 matter, and then replaced in the turpentine bath and afterwards 

 mounted as above. 



