BY R. GREIG SMITH. 123 



It died towards the end of the second day. The two mullets 

 developed reddish discoloured patches at first on an area around 

 the point of inoculation and afterwards further forward on that 

 side and also on the other. The scales fell off these places and 

 the body became swollen. The smaller mullet died in 45, the 

 larger in about 100 hours. 



In the post mortem examination of the trevally the muscle 

 beneath the epidermal scar was seen to be of a cream-white colour 

 and brittle, both in appearance and texture like boiled cod roe. 

 Portions of this when pressed between cover-glasses were found 

 to be swarming with motile bacilli. The muscle on the side 

 reverse to that inoculated was apparently healthy and free from 

 bacteria. The blood vessels were congested. The peritoneal 

 cavity contained a quantity of yellow serous fluid in which floated 

 large white cheese-like masses. The liver was pale and mottled, 

 the gall bladder distended, the stomach slightly congested. The 

 kidney*, spleen and heart were apparently normal. The intestine, 

 especially in the posterior portion, was much congested. A 

 number of inoculations were made on sloped agar and these 

 showed the organisms in pure culture in the heart blood, the 

 gangrenous muscle near the site of inoculation and the apparently 

 healthy muscle on the reverse side. The liver and peritoneal 

 fluids gave negative results. 



The smaller mullet showed very few lesions. The intestine 

 was congested and filled with gas, the liver was dark and mottled. 

 The other organs were apparently normal, and the muscle, 

 although discoloured near the point of inoculation, did not show 

 the localised gangrenous appearances of the other fishes. Pure 

 cultures of the organism were obtained from the liver, the heart- 

 blood and the muscle from the inoculated as well as the reverse 

 side. 



The larger mullet showed several hemorrhagic areas over the 

 surface of the epidermis. The scales had fallen from places, 

 revealing patches of a purplish to grey-blue tinge with reddish 

 streaks. The muscles had three gangrenous spots, one at the 

 site of inoculation, another further forward on the reverse side 



