BY R. GREIG SMITH. 453 



method of staining. No spores were observed. It grows well 

 at 37°. 



Agar plate. — Circular, white, moist glistening, raised colonies 

 are formed. When magnified they are seen to have a smooth 

 edge; the internal structure is slightly marbled. The deep 

 colonies are irregular and rough. 



Agar stroke. — A luxuriant, white, raised layer with smooth 

 iridescent edge grows along the stroke. The edge becomes lacerate. 



Gelatine plate. — White, circular liquefied areas are produced. 

 Under a sixty-fold magnification, the subsurface colonies are seen 

 to be irregular, dark brown and apparently flocose, the surface 

 colonies have a flocose centre and a clear margin. 



Gelatine stab. — At first the stab is slightly crateriform but 

 becomes funicular. The liquefied gelatine becomes turbid and a 

 white precipitate forms. No film appears and the turbidity 

 persists. 



Glucose-gelatine. — No gas is produced. 



Bouillon. — The medium becomes turbid, a slight film is formed 

 as well as a pale white precipitate. Traces of indol are produced. 



Milk. — The casein is coagulated and the reaction becomes acid. 



Potato. — There is formed at first a pale ochre moist glistening, 

 raised growth, which becomes yellowish-brown, irregular and 

 greasy. The layer continues to rise from the surface of the 

 potato, and when growth stops it is mesenteric, dull, and of a light 

 drab colour. The potato is darkened. 



Nitrate-bouillon. — The nitrate is reduced to nitrite. 



The affinities of this organism are with Bact. Fairmontensis , 

 Wright, Bac. aquatilis communis, and Bact. No. Jfi, Conn. From 

 the first named it differs in the appearance of the gelatine colonies 

 and the growth in gelatine stab culture as well as in the growth 

 upon potato. Bac. aquatilis communis is considered by Kruse to 

 be a Bact. fluorescens liquefaciens which has lost its power of pro- 

 ducing fluorescence. It therefore differs from this organism in the 

 reaction of the milk when coagulated. Bac. aquatilis communis 

 forms a yellowish-brown or reddish layer on potato and is never 



