458 BACTERIAL FLORA OF THE SYDNEY WATER SUPPLY, 



colonies. It can withstand limited amounts of alkali, since it 

 was obtained upon agar containing - 5 % sodium carbonate. 



Bacterium subflavum teres, n.subsp. 



Shape, etc. — The organisms measure 0-6 : 1*5 /z. Shorter and 

 longer forms occur. The cells are oval and are actively motile. 

 They are not stained by Gram's method. No spores were observed. 

 It grows well at 37°. 



Agar plate. — The colonies are circular, raised, moist glistening 

 and buff-coloured. When magnified, the structure appears 

 granular and the edge smooth. The deep colonies are opaque, 

 irregular and rough. 



Agar slope. — -The growth is moist glistening, buff-coloured, 

 raised and viscous; it spreads out at the base and the colour 

 becomes deep yellow. 



Gelatine plate. — The airogenes-like colonies are cream-coloured 

 and circular, moist glistening and slightly raised. When magni- 

 fied, the deep colonies are seen to be circular and zonate; the 

 surface colonies are circular, finely granular and brownish-yellow 

 in colour. 



Gelatine stab. — A scanty filiform growth is crowned by a raised 

 nail-head, which spreads regularly. The convex growth is light 

 buff and moist glistening. 



Glucose-gelatine. — No gas is developed. 



Bouillon. — The medium becomes slightly turbid and there is 

 formed a filamentous precipitate and slight film. A slight indol 

 reaction was obtained. 



Nitrate-bouillon. — Nitrate is not reduced. 



Milk. — The reaction becomes alkaline and there is no coagu- 

 lation even upon warming. 



Potato. — A canary-coloured, moist glistening, spreading, flat 

 layer is formed. 



The organism grows moderately quickly, and in this respect 

 differs from its near allies Bac. aureus, Adametz, and Bac. auran- 

 tiacus, Frankland. The cerogenes type of the gelatine colonies 



