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poultry raisers will feel timid about injecting into valuable birds 

 a solution which they know contains the disease germ, it may be 

 safer for them first to learn to administer the vaccine and satisfy 

 themselves of its beneficial effect, before they attempt to prepare 

 it. For those who feel this way about it, the following is sug- 

 gested. 



COLLECTING AND FORWARDING SCABS FOR VACCINE. 



Until such a time as poultry raisers in the Territory feel confi- 

 dent of the value of the vaccination treatment, the Territorial 

 Veterinarian will prepare and, whenever possible, return by first 

 mail the vaccine made from scabs forwarded to his office for this 

 purpose. 



When care is taken promptly to isolate all affected birds, the 

 disease as a rule spreads slowly, and as it is necessary that a suffi- 

 cient quantity of crusts or scabs should form in order to obtain 

 material for the treatment of the entire flock, there is little dan- 

 ger of heavy losses even if four to six days will be required for 

 the forwarding, preparation and return of the scabs and vaccine. 



The best scabs, and in fact the only ones to use for vaccine, are 

 those which form on the comb, wattles and the skin of the head. 

 In no case use the exudate which forms in the eyes, nostrils or 

 mouth. Place the scabs in a small bottle, previously cleaned 

 with boiling water and drained until dry. At least one heaping 

 teaspoonful of scabs will be required for the vaccination of a 

 flock containing from 75 to 100 birds, but as this quantity pro- 

 vides for two injections, (the second injection following the first 

 after five to seven days), it is not necessary that the entire amount 

 should be forwarded at once. For a flock of 25 to 30 chickens, a 

 much smaller quantity will suffice for the first injection, and as 

 the treatment does not immediately stop the further development 

 of sores or tumors, a sufficient quantity of scabs for the second 

 treatment will usually develop during the interval between the 

 two injections. The second crop should, therefore, be forwarded 

 not more than five to seven days after the first was sent. 



Wrap the bottle well and place it in a small box or mailing 

 case plainly addressed to the Division of Animal Industry, Board 

 of Agriculture and Forestry, Honolulu, and send it by special de- 

 livery. Full information concerning the number, class, breed and 

 age of the birds to be treated must accompany the shipment, as 

 well as plain instructions as to where the vaccine should be re- 

 turned. 



METHOD OF PREPARING THE VACCINE. 



The method of preparing the vaccine, vaccination, precautions 

 to be observed, and treatment, evolved by Dr. J. H. Beach of the 

 University of California, and the ones which have been followed 

 here, are substantially as follows: 



