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One-half gram of chicken-pox scabs to 100 cubic centimeters * 

 of physiological salt solution is the proportion used. The scabs 

 are first weighed out and ground in a sterile mortar with a small 

 amount of the sterile salt solution until they are pulverized. This 

 material is then filtered through absorbent cotton into a sterile 

 flask or bottle and the remainder of the salt solution poured 

 through the filter so as to wash out as much of the pulverized 

 material as possible. The flask is then stoppered, placed in a 

 water bath and heated at a constant temperature of 55° Centi- 

 grade t for an hour. The vaccine is now ready for use. It is 

 very essential that the vaccine be used as soon after preparation 

 as possible. Since no preservative is used it will deteriorate if 

 allowed to stand. 



METHOD OF VACCINATION. 



The vaccine is administered by injecting it beneath the skin 

 with a hypodermic syringe. Two doses of one cubic centimeter 

 each are given five to seven days apart. The most convenient 

 place for administration is beneath the skin of the side under the 

 right thigh, the skin at that point being comparatively free from 

 feathers. The left wing is held back, the fowl laid on its left side, 

 and the right wing and leg and feathers held back with the last 

 three fingers of the left hand. The exposed skin is then cleansed 

 with a piece of cotton saturated with disinfectant solution (2 

 percent solution of compound solution of cresol) and picked up 

 with the thumb and forefinger of the left hand. Then with the 

 right hand the syringe needle is inserted beneath the skin and the 

 proper dose injected. A syringe of one cubic centimeter capacity 

 is well suited for this work, when small flocks are to be treated. 

 (Ask your druggist for Cutter's Tuberculin Syringe.) 



PRECAUTIONS TO BE OBSERVED. 



1. The vaccine being in an unpreserved condition will soon 

 decompose and become unfit for use. Therefore, if possible, it 

 should be used within three days. If old, decomposed vaccine 

 is used bad results will follow. 



2. Vaccine should be kept in a cool place, on ice if practicable, 

 until used and only one bottle opened at a time. 



3. A small, wide-mouthed, covered vessel, such as a quarter- 

 pint milk bottle or a jelly-glass, should be provided as a vaccine 

 container from which to fill the syringe. This should be sterilized 

 by boiling before it is used and should be kept covered at all 

 times except when the syringe is being filled. 



4. The syringe should be sterilized by boiling, or by soaking 

 for several minutes in a ten per cent solution of compound solu- 

 tion of cresol, followed by rinsing with boiled water. 



* One cubic centimeter=approximately 15 drops, 

 t 55° centigrade=131° Fahrenheit. 



