165 . . ' 



move all the infected sod to a depth of from 4 to 6 inches, which 

 was heaped on top of the places where the carcasses had been 

 burned, and the mound thoroughly sprinkled with a 1 to 100 

 solution of corrosive sublimate. The graves of the animals 

 which had been buried were located as far away as possible and 

 the same procedure applied to the surface over the graves. A 

 larger amount of sod and dirt would be heaped on top of the 

 graves, in order to prevent the same from sinking after the de- 

 composition of the animal and the forming of a depression, and 

 eventually a pool over the grave. 



The serum vaccine was received on April 25, all available 

 chutes having in the meantime been reconstructed for vaccination 

 purposes. From that time on all the animals in the infected 

 pasture were passed through the chute and injected with 10 c. c. 

 anthrax serum on one side of the neck and 1 c. c. anthrax vaccine 

 on the other side, the two injections being made simultaneously 

 by one operator on each side of the chute. Unfortunately strong, 

 cool rains prevailed nearly every night which may have been the 

 cause of the death of a number of the animals following the vac- 

 cination. In any case the vaccine failed to stop the loss to the 

 extent which had been hoped for. There can be little doubt 

 that had the vaccine not been applied the rate of mortality would 

 have continued to increase instead of being greatly reduced. 



By the end of the month all of the vaccine had been used up 

 and new cases of anthrax had begun to appear in heretofore un- 

 infected districts. On April 30 the total losses were estimated 

 at about 90 head. At this time a second shipment of 1000 doses 

 of serum vaccine was received and it was decided to continue with 

 the vaccination until all animals on the ranch had been treated. 



The further history of the outbreak will be dealt with in the 

 May report of this Division. 



The following rule and regulation had in the meantime been 

 promulgated by the Board and published in the daily papers of 

 Honolulu and Kauai : 



RULE X 

 Division of Animal Industry. 



"Rule and Regulation of the Board of Commissioners of Agri- 

 culture and Forestry concerning the quarantine of all domestic 

 animals on the Island of Kauai, on account of Anthrax and 

 Hemorrhagic Septicemia. 



"Owing to the fact that disease known as Anthrax or charbon, 

 and Hemorrhagic Septicemia, which affect most classes of domes- 

 tic animals, and the former of which may be transmitted to man, 

 have made their appearance on the Island of Kauai, it is hereby 

 ordered : 



"Section 1. That there is hereby declared a quarantine of the 



