235 



after injection, so that in most positive cases it is possible to come 

 to a conclusion within two to four hours after injection. At the 

 same time, the method is so simple that even a stable boy can 

 interpret the results, if he has once seen it applied, or if it has 

 only been explained to him. This test consists in the injection 

 into the hide on the side of the neck of two to three drops of the 

 official P)ureau of Animal Industry mallein. The injection leaves 

 a small, well-defined swelling", the size of a pea, which soon 

 begins to enlarge, so that, within an hour or two, it has reached 

 the si?e of a nickel or, in positive cases, a quarter. If the disease, 

 glanders, is not present in the system the swelling will from then 

 on remain stationary, or only enlarge very little more during the 

 next twenty-four hours, remaining" cold and not sensitive to touch. 

 But if the disease is present the swelling will continue steadily to 

 enlarge, sometimes at the rate of half an inch in diameter per 

 hour, and at the same time becoming hot and extremely sensitive 

 to touch. After ten to twelve hours the swelling may be six to 

 ten inches in diameter and raised from one to three inches above 

 the surrounding tissues, with sharply-defined margins. It is 

 easily seen that such a reaction is vastly more diagnostic than one 

 which, like the new official eye test, depends upon the quantity 

 of tears discharged from the injected eye, especially when both 

 eyes are already inflamed and running as a result of a cold, ship- 

 ping fever, influenza, strangles, or simply irritation by strong sun- 

 light or flies. 



That our intradermal mallein test would be adopted, were it 

 presented before the congress in question, there can be no doubt, 

 the results obtained at the .last outbreak of the disease in this 

 Territory — the Waipio Valley outbreak — having demonstrated its 

 value in 36 cases. In at least twenty of these cases the ophthalmic 

 test could not have been used on account of sore eyes. However, 

 both of these new methods of testing have been reported to the 

 board in many previous communications, and the present state- 

 ment was v/ritten only with a view to emphasize the importance 

 of bringing them before the public in an effective manner. For 

 this reason also has no publication been made in any veterinary 

 or scientific magazine or periodical up to this date. 



In regard to the interrogation from the Board of Supervisors, 

 through Dr. Wayson, as to the statement made by me in my last 

 report to the effect that the local milk inspection "is a farce," I 

 have been requested not to publish my reasons for this statement, 

 but allow the city and county physician to look into the matter, on 

 the basis of such information as I might provide him with on the 

 subiect. I would therefore respectfully request that, for the sake 

 of future cooperation, I be allowed to deal directly with the city 

 and county physician on this subject in order to assist him in 

 straightening up the situation, w^hich without doubt requires both 

 disciplinary and corrective measures. The very fast that infan- 



