Tech. F:2 



tissue filter materials and final containers for the nematodes; the 

 first using paper cups, the second using tubes formed from polyethylene 

 films. 



Caveness, Fields E. , and Harold d. Jensen, 1955. Modification of 

 centrifugal -flotation technique for isolation and concentration of 

 nematodes and their eggs from soil and plant tissues. Proc. Heljm. 

 Soc. Wash. 22(2): 87-89. 



Miller, Patrick M., 1957. A method for the quick separation of nema- 

 todes from soil samples. Plant Dis. Reptr. I|1(3):19U. The centrifuga- 

 tion-flotation technique as described is applicable to rather small- 

 sized soil and plant samples but is of particular interest as a means 

 for recovery of nematode eggs. The further modification of the tech- 

 nique by Miller consists of screening the nematodes from the sepairating 

 sugar solution instead of alla^^ing them to settle, this reduces the 

 overall time of the process considerably. 



Mijnderman, G., 1956. New techniques for counting and isolating free- 

 living nematodes from small soil samples and from oak forest litter. 

 Nematologica 1(3) :2l6-226. Describes two techniques for use In 

 investigating nematodes in small-sized soil samples. One involves 

 staining of the nematodes in samples where total counts of nemas are 

 desired. The other technique is a combination of centrifugation and 

 flotation of the nematodes using magnesium sulphate solution. This 

 method may have application for recovery of eggs from samples and a 

 way for mechanization of the processing is illustrated. 



Manipulation of Nematodes 



Ford, Harry W., 1957. A source of controlled vacuum for pipetting 

 nematodes. Plant Dis. Reptr. Ul(2):89-90. A simply constructed 

 device for applying a very weak suction to a pipette for removing 

 individual nematodes from a dish. One ingenious feature of the device 

 is that pipetting action will stop automatically when the tip is lifted 

 above the surface of the water because surface tension of the water at 

 the tip of the pipette is greater than the vacuum applied to the pi- 

 pette. 



Pathogenicity Experimental Work 



Mountain, W. B., 1955- A method of culturing plant parasitic nema- 

 todes under steril conditions. Proc. Helm. Soc. Wash. 22(1) :U9-52. 

 A technique for rearing plant-parasitic nematodes under aseptic con- 

 ditions on root cultures. 



■K- -;;- •«• 



Fenwick, D. W., 1956. The production of sterile viable larva? of the 

 potato root eelworm, Heterodera rostochiensis . Nematologica 1(h) :331- 

 336. A simrplified technique for obtaining eggs and Inrvae which may 

 be adaptable to Hcloidogyne and other plant-parasitic forms. 



