Tech. A-.r, 



sinlc-mats or by wooden-slat racks in the bottom. 



A useful addition is to have either a separate water outlet, to 

 which a piece of tubing can be fastened, or a spray-hose sink 

 attacliment. These are used in directing a strea/n or spray of 

 water on the sieves and for flushing soil out of the pails and 

 sink. It has been found that an Inexpensive aerorator device on 

 the faucet will result in stimulated activity of the nematodes 

 and is, therefore, highly recommended for installation at any 

 sink where samples are to be processed or where water for the 

 Baermann funnels is obtained. 



8. Soil disposal is a matter requiring special consideration, if 

 there is a chance that nematodes which are new to the locality 

 may be brought in and become dispersed. Soil processing arrange- 

 ments having settling tanks, sumps, or pits can be treated peri- 

 odically with a poisonous chemical. This will reduce the hazard 

 of overflow or run-off water carrying viable nematodes. The 

 accumulated soil can be treated by chemicals, including soil 

 fumigants. 



In laboratories where the processed sample residues are not passed 

 into special drains, these materials can be accumulated in pails 

 and carried out of the lab to special locations for contaminated 

 materials. Nematodes in these residues can b e killed by steam 

 sterilization, fumigation, or, in some cases, by retaining in such 

 a manner that plant-parasitic nematodes would be starved, des- 

 sicated, or subjected to lethal exposures of light, heat, or cold. 



II. Equipment and Mat-?rial for the Field 



A. A shovel is needed for digging plants v^xhen field examinations of the 

 root systems are necessary. The roots should be dug so that the 

 small roots remain intact. Remove the plant and its adhering soil 

 ball from the ground, and knock the soil from off the roots onto the 

 shovel blade. This assures getting the soil which was in closest 

 contact VJith the roots ard thus having the highest concentration of 

 nematodes of interest. 



B. Soil sampling tubes and soil au gers can be used to get samples from 

 various depths in the ground and are an excellent means of quiclcly 

 getting numerous, relatively small samples for consolidation into a 

 larger, single sample, such as may be desired for survey work. The 

 soil tube is preferable to the soil auger in most localities, as the 

 soil sample is retained in the tube and can be readily stripped out 

 of the slot in the side of ^ the tube and caught in the sample con- 

 tainer. Soil tubes which require tapping or a rami-od for removal of 

 the soil core are not as satisfactory as the above mentioned slotted- 

 type. Soil augers may have to be used where the soil is rocky or 

 very hard. The disadvantages of the auger are its slowness in use 



