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EQUIPMENT AND MATERIALS /fe" >'^'^* '^*'.. 'i* 



I. Equipment and Materials for the Laboratory 

 A. Optical Equipment: 



1. Binocular sterescopic microscope of a type possessing a pedestal 

 base or otherwise mounted so as to permit transmitted illumina- 

 tion of the specimens. A range of magnifications from low to 

 high powers is desirable. 



2. Honobjective microscope equiped with a mechanical stage, sub- 

 stage condenser, and objectives ranging from low to high magni- 

 fications (oil immersion). A set of oculars with powers from 

 about $1 to l^X and interchangeable binocular body tube are 

 useful additions. Achromatic lenses are satisfactory for most 

 work, but an apochromatic oil immersion objective with compensat- 

 ing eyepieces is much to be preferred for critical examinations 

 requiring maximum resolution at high magnification. 



3. Illumination froia an electric illuminator of a condenser type 

 capable of providing the Koehler system of illumination is 

 desirable, if detailed microscooic examinations of the nematodes 

 are to be" made . 



U. Measurements of nematodes and their parts v/ill require an ocular 

 micrometer disc. A filiar micrometer eyeoiece is a useful acces- 

 sory for very accurate measurements, but it is not a necessity. 

 Calibration of the ocular disc or the filiar micrometer will 

 require access to a stage micrometer, 



5. Camera lucida apparatus is also useful, if not necessary. Most 

 nematoiogists dispense with the small mirror provided and instead 

 use a large-sized, front- surfaced mirror. A source of these 

 mirrors, which are made to size and stocked just for this purpose, 

 is the Pancro Mirrors Co. Inc., 2958 Los Feliz Blvd., Los Angeles 

 39, California. The item is listed as camera lucida mirror, 6X9 

 inches, 1/8 inch thick mirror- quality glass, front-surfaced Pancro 

 coating. The mirror is mounted on a piece of wood, and a very 

 satisfactory assembly for the mirror can be made from standard 

 Flexiframe supports obtainable from scientific supply houses. 



B. Slide Making Materials: 



1. Microscope slides of the 3X1 inch size are most used. The best 

 quality, non-corrosive slides should be used for permanent slides. 

 Clinical grade or other lees highly finished or selected grades 

 are satisfactory for routine work. A special metal slide which 

 holds the nematode specimens moiinted between two coverglasses is 

 obtainable. This device permits study of both sides of the 



