Tech. B:l 



OUl'LIi^-E ?0K PROCBSoING SAMPLES 



1. The complete and, therefore, the best sample consists of plants and 

 soil from the plant's rhizosphere. 



2. The samples shoidd be reasonably representative of the condition 

 involved, whether it is only a part of a single plant, a row or 

 spot, or an extensive planting. 



3. The samples should be maintained in such a manner that nematodes 

 present will not be dead from overheating or dessication upon 

 arrival at the laboratory or before processing. 



Soil and Plant Samples 



I. Soil with:' 



Cyst forming nematodes 



A. Dried 



Recovery of "floaters" 



B. Not dried 

 Decanting &. Sieving 



Non-cyst forming nematodes 



A. Baer-mann funnel 



B. Decanting c^ Sieving 



C. Combination of both 



D. Direct observation 



E. Seinhorst Slutriator apparatus 



II. Plant with: 



Endo- and/or Ectoparasitic nematodes 



A. Direct examination 



itoots and foliage for nematodes 

 and symptoms of nematode damage. 



B. Isolation of nematodes 



1. Teasing out 



2. Soaking 



3. Baermann funnel 



U. Seinhorst extraction technique 



5. Incubation method 



6. Blendor and Sieving 



C. Staining in situ 



1. Osmic acid methods 



*2. Lactophenol methods 



3. Bromphenol or bromthymol method 



U. Aceto-osmimn method 



