(Not included in copyright.) 



Tech. E:8 



MODIFICATION OF RAPID METHOD FOR MOUNTING NH^IATODF^ IN GLYCERIN 

 Dr. A. D. Baker, Department of Agriculture, Ottawa, Canada 



With Lactophenol the proportions would be as follows: 



Series - I II III IV 

 Lactophenol 75 50 25 12.5 



Glycerin 25 50 72 85.0 



Formol 3 2.5 



V 



From fixative to Lactophenol and then through the above series to" pure 

 Glycerin. About 10 minutes in each of these mixtures at a temperature 

 of 50 - 55° F. 



In preparing these mixtures, it is convenient to have a stock solution 

 of equal parts of Lactic Acid and Phenol (crystals) available. This 

 should be kept in an amber bottle. Lactophenol and the above mixtures 

 should also be stored in amber bottles. 



Stain may be included with the Lactophenol, as described for cotton 

 blue staining (0.01 per cent) by Franklin & Goodey in 19h9. In making 

 up these mixtures, add the stain to the distilled water used in making 

 up your Lactophenol, or dissolve the dry stain in Glycerin (with heat), 

 femember that most stains are soluble in Glycerin. Thus, if some fad- 

 ing of the color is not to occur, it is sometimes necessary to faintly 

 "tint" the mixtures, as well as the final mounting medi\im. It is also 

 important to note that all nematodes do not stain equally well with the 

 same stain. Cotton blue is a useful stain, but one has to be careful 

 not to overstain. Other stains that may be tested for particular needs 



