Tech. EiE" 



12. Bring the head to perfect perpendicular by carefully pushing on the 

 edge of the cover-glass. This is first done under the binocular and 

 later, if necessary, perfected under the high power. The wax will 

 hold the cover-glass in place, yet allow for the necessary slight 

 movements necessary to bring the head into perpendicular. (Staining 

 the nematode before cutting off the head aids in locating and orient- 

 ing the face view. Acid Puchsin, either in Goodey's formula or in 

 fixative, may be used. The stain usually fades out withing 2k hours.) 



