534 i>R- w. 0. MOOR, 



cohol to each litor of urine. In a few minutes tlic alcohol can be decantered, 

 thiis leaving in tlie rocipicut tlie chlorides, phosphates, sulpliates, the uric 

 acid, Creatinine and the principal coloring matter of the urine, a yelb)wis]i- 

 brown, résinons substance, while the alcoliol contains ureine, iirea and 

 some coloring matter. The alcoholic liquid is well shaken for one minute 

 with powdered animal charcoal, 30 gr. charcoal to each 200 cbcm. 

 of alcohol, and then filtered; the charcoal retains tlie re.st of the coloring 

 matter and sorac other admixture, especially Chloride of Sodium ') The filter 

 is washed out once or twice with absolute alcohol and then the whole alco- 

 holic filtrate is evaporated at 40 — 45° C. until every trace of alcohol or 

 water bas disappeared; this is usually the case, when the formation of urea 

 crystals becomes uoticcable at a température of 40° С ^). 



We now place the récipient upon ice and after a few minutes we add 

 to the usually solid mixture of ureine and urea a small amount of icecold, 

 absolute alcohol, — about twice as much alcohol as ureine -♦- urea. The 

 method of separating ureine from urea is based upon the fact, that ureine 

 is more readily soluble in alcohol at 0° C. than is the case with urea cry- 

 stals, so that by gently mixing the absolute, icecold alcohol with the ureine 

 -+- urea nearly the entire amount of ureiue eau be dissolved, while a con- 

 sidérable portion of the urea crystals remains on the bottom of the récipi- 

 ent. The yellowish alcoholic liquid containing the ureine and also some urea 

 is decantered and again evaporated at 40 — 45° C. ; the yellow liquid re- 

 maiuing after the evaporation of the alcohol is left auew at 0° C. until a 

 sufficient amount of urea crystals hâve separated, whereupou it is treated with 

 alcohol like before. Finaljy we pour the ureine into a long, narrow testtube, 

 which is left in ice for 24hours; at the end of this time the ureine is either 

 filtered, if somewhat hazy, or simply decantered, if perfectly limpid. 



The ureine obtained by this method is au oily, not especially dense 

 liquid, whose specific gravity is 1250; it bas a beautiful, goldenyellow 

 color, but does not contribute towards the yellow color of the urine, as one 

 cubic-centim. of ureiue diluted with 20 cbcm. of water already gives a 

 perfectly colorless solution. 



While it was not ту purpose to ascertain the exact chemical consti- 

 tution of ureine, yet two facts bave attracted my attention: 



1) It is a nitrogenous body. 



2) It contains five or six times more carbou than does urea. This is 

 a fact of great importance to the physiologist, for it explains, why a certain 



1) By washing out the charcoal with water wo can obtaiii theae substances. 



2) The urea crystals form a network, to which the ureine adhères. 



Фив.-Мит. стр. ]«3в. 6 



