QUANTITATIVE ESTIMATION OF THE ALKALOIDS. 71 
a 10-centimeter (4-inch) capsule, 30 cubic centimeters (1 fluid- 
ounce) of hot water are added, and when the lime is slaked, the 
mixture is stirred, and 5 grams (77.16 grains) of the powdered 
cinchona’ are added, the mixture very thoroughly stirred, and 
digested in a warm place for a few hours, or over night. The 
mixture is then dried, at a low temperature, on a water-bath, rubbed 
to powder in the capsule, and transferred to a flask of 100 cubic 
centimeters (3.3 fluidounces) capacity, and 25 cubic centimeters 
(0.8 fluidounce) of amylic alcohol added. The flask is afterward 
corked, and digested in a water-bath at a boiling temperature, 
with frequent, vigorous shaking, for four hours. It is then allowed 
to cool, and 60 cubic centimeters (2 fluidounces) of stronger ether, 
spec. grav. 0.728, added, and again shaken vigorously and fre- 
quently during an hour or more. The liquid is now filtered 
through a double filter of 10 centimeters (4 inches) diameter into 
a flask of 150 cubic centimeters (5 fluidounces) capacity, and the 
residue transferred to the filter. The flask is rinsed, and the 
rinsings brought on to the filter with a mixture of 10 volumes of 
amylic alcohol and 40 volumes of stronger ether, and the residue on 
the filter percolated with 15 cubic centimeters (0.5 fluidounce) of 
the same mixture, added drop by drop from a pipette to the edges 
of the filter and surface of the residue. The residue is afterward 
returned to the flask from whence it came, 30 cubic centimeters 
(1 fluidounce) of the amylic alcohol and ether mixture added, 
shaken vigorously for 5 minutes or more, and the whole returned 
to the filter, and the residue again percolated with 15 cubic centi- 
meters of the menstruum, applied drop by drop from a pipette, as 
before. The filter and residue are now put aside, in order that 
the latter may afterward be tested in regard to the degree of 
exhaustion. | 
‘The ether is now boiled off from the filtrate in the flask by means 
of a water-bath, taking great care to avoid the ignition of the ether 
vapor, and also to avoid explosive boiling, by having a long wire 
in the fask. When boiled down as far as practicable in the flask, 
the remainder is transferred to a tared capsule of 10 centimeters 
(4 inches) diameter, and the evaporation continued on a water- 
bath until the contents are reduced to about 6 grams (92 grains). 
This is transferred to a flask of 100 cubic centimeters (3.3 fluid- 
ounces) capacity, rinsing the capsule with not more than 4 cubic 
centimeters (64 minims) of amylic alcohol, and adding the same 
to the contents of the flask. 6 cubic centimeters (96 mimins) of 
water and 4 cubic centimeters (64 minims) of normal solution of 
1 A previous moistening of the bark with ammonia is also recommended, 
