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26 



2nd poi-tion were allowed to stand for 2 days in darkness in a nutrient saline 

 solution [1 gr. MgSO^-\- 1 gr. KH^PO^-^rCaSü^ per liter aq. destil.] the seedlings being 

 placed on paraffined gauze, which was stretched over the nutrient solution, of 

 which half a liter was used for each portion. The plants were covered with a 

 bell-jar of 7'6 liter's capacity. The 3rd portion was treated as the second, yet a 

 receiver with 5 c. c. ether was placed under the bell-jar. [As the vapours of ether 

 are not only absorbed by the air but also by the water of the nutrient solution, 



5 

 the strength of the ether represents -tt^t nr^r no '^ ^'^^ ^'^- ^'her per liter air]. 



The cotyledons were immediately cut off the plants of the 4th, 5th and 6th por- 

 tions. The 4th portion was immediately analysed, while the 5th portion was treated 

 as the 2nd, and the 6th as the 3rd portion, after which process they were analysed 

 as to ascertain the amount of nitrogen of the proteids by Stutzer's method. The 

 following results were obtained: 



I. 



Shoots 



Cotyledons 

 Shoots alone. 



Shoots 



Cotyledons . . 

 Shoots alone 



1. 

 Control portion 



89-80 mgr. N of proteids 

 310-74 - - - 



89-23 - - 



2. 

 2 days with- 

 out ether 



3. 



2 days 



■with ether 



94-00 mgr. 

 232-64 - 



88-94 - 



110-72 mgr. 

 251-04 - 



88-25 - 



3. 



92-15 mgr. N of proteids 100-12 mgr. I 115-99 mgr. 

 301-24 — - - 225-34 - I 239-86 — 



94-54 - - - i 90-00 - I 89-98 - 



Of these results Zaleski concludes that etherization produces more rapid trans- 

 location than under normal circumstances of the proteids from the cotyledons to the 

 shoot. Either the proteids pass into the shoot, or the proteids are formed in the shoot 

 by the amides which are conveyed from the cotyledons. [Zaleski 1900, p. 292 seq.]. 



Yet it seems contradictory to assume that the translocation may be accelerated 

 simultaneously with a retardation of the formation of substances suitable for the 

 mobilization. ' The data at hand are however quite insufficient to deduce any 

 conclusions from concerning the rapidity of translocation, as it is necessary to 

 know separately the total amount of nitrogen of the cotyledons and of the shoots. 

 Unfortunately Zaleski has not examined the amount of these substances. 



To investigate the matter I have made some experiments for which I used 

 the same method as Zaleski; I have however estimated the total amount of nitrogen 

 in the respective organs instead of the amount of nitrogen of the proteids. The 

 following results were found : 



' That the nitrogenous compounds are translocated as amides and not as proteids may hardly 

 be contradicted. [Czapelt II 190.5, p. 159]. 



