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247 



CHAPTER V. 



Experiments on the Effect of Etherization on Buds of Acer Pseudoplatanus. 



1. Equally developed buds of Acer were cut from the tree (April 1907) and 

 divided in seven portions as uniformly as possible, each portion containing 15 

 buds. None of the buds had begun to open either before or after the experiment. 

 The first two parallel-portions, which were crushed with alcohol, as soon as they 

 had been cut off the tree, showed that the material was uniform to a high degree. 

 All the other portions were allowed so stand in a dark room in a constant tem- 

 perature of c. 10° C. for two days with or without ether, as may be seen by the 

 table; after this exposure they were crushed as usual. The following results were 

 obtained by the analyses: 



Absolute 

 quantities 

 of ether 



Weiglit 

 of buds 



N per 20 c. c. 

 of the filtrate 



Irom the 

 tannic acid- 

 precipitate 



N of 

 araides in 

 per cent 

 of the 

 original 

 amount 



■ ; Directly crushed [\ 



3. 2 days without etiler I nil. 



i. 2 — with Ü-5 c. c. ether per liter i 7"00 c. c. 



5. 2 — — 0-75— — ^ J! 10-50 - 



6. 2 - — 1-0 - — - i 14-00 - 



16-84 grs. 



15-28 - 

 15-46 - 

 16-69 ~ 

 16-70 - 

 16-44 - 



1-25 — 



- i; 17-50 - '15-51 - 



8-80mgrs. j\jQQ.Qo 



8-75 

 10-80 

 8-30 

 8-65 

 9-70 

 9-75 



r 



123-01 — 



94-53 — 



98-54 - 



110-48 - 



111-05 - 



\ After the exposure 

 /the buds were fresh. 

 ^ After the exposure 

 [ the buds wei-e 

 J discoloured 



These results agree with those Johannsen arrived at in the same manner 

 [Johannsen 1897, p. 73] : 



1. 2. 3. 4. 5. 6. 7. 



89 mgrs. 91 mgrs. UOmgrs. 9-5 mgrs. 98 mgrs. 108 mgrs. 11-0 mgrs. iV of amides. 



90 

 (The numbers are multiplied with 2 in order to obtain a survey). The fact 

 that number 4 and 5 of my experiments show somewhat larger effects than those 

 of Johannsen, must be explained by the circumstance that the absolute doses of 

 ether, which I have used , were a great deal larger than those employed by 

 Johannsen, who used jars of 4 litres capacity, while I have used jars of 14 litres 

 capacity for the process of etherization. Concerning this experiment and similar 

 ones made on buds of beeches, on elderberries and on wounded potato-tubers I 

 quote from the above mentioned student who says: "De vise, at Beskadigelse kan 

 foraarsage en Forøgelse af Amid-Kvælstoffet, der er stærkere end den Forøgelse, 

 som Narkosen fremkalder; og tillige ses det, at Ætherisering, resp. Chloroformering, 

 i det mindste med svagere Dosis, afgjort nedstemmer Saar-Virkningen." ["They 



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