﻿248 



36 



show that wounding may cause an increase of Ihe amides, larger than the in- 

 crease produced by the narcosis; they prove too that etherization, respectively 

 chloroforming, retards decidedly the effect of wounding, always when small doses 

 are used"]. The considerable increase of amides, shown in 3, is hardly to be 

 referred solely to the wounding, as it must be remembered that the buds have not 

 only been exposed to wounding, but they have also been exposed to darkness; 

 furthermore they have been cut off from all absorption, respectively translocation 

 of materials; consequently we must give consideration to all these factors. For 

 further investigation of the matter the above mentioned experiment was supple- 

 mented with the following: 



II. Buds^ 5 X 14 large, as similar as possible regarding size and develop- 

 ment, were chosen from the same tree from which material for the above men- 

 tioned experiment had been taken. The first, second and third portions of these 

 buds were immediately cut off (April 27th 1907), while the 4th and 5th portions 

 remained on the tree till April 29th, when these too were removed and crushed 

 for analyses. The buds of the 4th portion were split on April 27th; they were 

 thus exposed to injury during the two days they still remained on the tree. The 

 first and second portions were crushed for the analyses directly after being cut off 

 from the tree, while the buds of the third portion were put two and two together in 

 test-tubes, which were closed with gauze to prevent the buds falling out; and to 

 prevent rain getting into the test-tubes they were infixed in inverse position on the 

 tree, where they remained till April 29th, when the buds were crushed. During 

 the experiment the temperature was in the middle of the days 14°, but fell during 

 the first night to 43°; the second night only to 9°: 



N of 20 e. c. of I N of amides i 

 Weight I tile filtrate from j p. c. of tlie 

 of the buds | the tannic- j original 

 acid-precipitate amount 



■ \ Directly crushed (April 27th) . . .' 



3. Cut off and split ; 2 days in test- 

 tubes on the tree 



4. Split Apnl27th. Cut off and crushed 

 2 days later 



5. Cut off and crushed April 2yth 



16-72 grs. 

 17-75 - 



17-35 - 



19-55 - 

 20-70 - 



8-55 mgi-s \\ 100.00 o;„ 

 8-20 — 1/ 



S-48 - 



101-50 — 



8-73 - ' 104-55 — 

 8-33 - 99-89- 



The buds were 



fresh after 



the exposure. 



III. On May 9th 7 x 13 buds, slightly more developed than those, used lor 

 the second experiment, were picked out and marked on the tree. The buds of the 

 first portion were immediately cut olT and crushed, those of the second portion 

 were cut off and exposed to darkness for two days in a tinpail in a room directly 

 in front of an open window; the buds were crushed on May 11th. The third por- 



The buds contained 197!t "(o of dry weight of which 5-44 "I. i of N. 



