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249 



lion stood likewise for two days in darkness in thie same place as the second one, 

 but these buds were exposed to a dose of ether of 01 c. c. per liter air. The 4th 

 portion was treated as the 3rd one, however without being exposed to darkness. 

 The ath portion remained for two days in light with 05 c. c. ether p. 1. The buds 

 of the 6lh portion were marked on May 9th on the tree with pieces of ribbon and 

 were picked and crushed two days later (May Uth). The buds of the 7th portion 

 were covered separately with black paper, impervious to light, and small test-tubes 

 were placed over them as a protection against rain ; thus the buds remained on 

 the tree from May 9th till May 11th, when they were cut oft" and prepared for 

 analyses. In the room, where the second and third portions had been placed, the 

 temperature varied between 9° and 13°, the temperature in the garden between 

 7-5° and 155°. 



III. 



Absolute 

 amount 

 of ether 



Weight of 

 {he l>uds 



N of 20 c. c. 

 of the filtrate 



from the 

 tannic acid- | 

 precipitate 



N of amides 

 in "/.I the 

 original 

 amount 



1. Directly crushed May 9th 



2. Cut off May 9th. Exposed to dark- 

 ness for 2 days 



;i. Cut off May 9th. 2 days in dark- 

 ness with 0-1 c.c. ether per liter. 



4. Cut off May 9th. 2 days in light 

 with 01 c.c. ether per liter 



.'). Cut off May 9th. 2 days in light 

 with 0-5 c.c. ether per liter 



(j. Cut off and crushed May 11th.. 



7. Exposed to darkness on the tree 

 for 2 days. Cut of and crushed 

 Mav nth 



1-4 

 14 

 70 



grs. 

 11-90 



1240 



11-66 



12-22 



12-98 

 17-02 



21-80 



ragrs. 

 5-26 



6-35 



6-93 



6-69 



5-65 



4-87 



5-15 

 [6-60] 



O/o 



100-00 



120-64 



131-72 



127-14 



10741 

 92-59 



97-83 

 [125-31] 



After exposure: fresh. 

 Afterexposure : frt'sh. 



discoloured. 



8 of the buds had 

 germinated. 



In III the amounts of amides were averagely smaller than in II and I, a 

 phenomenon which must be owing to the circumstance that the buds in the first 

 series were older than those of the second one and accordingly richer in water; 

 unfortunately, estimation of the dry weight has not been made with regard to the 

 buds in III. 



Of the results obtained it is possible to explain separately the effects of the 

 above mentioned factors. Injury alone has produced no effect (II 4), as the increase 

 of the amount of amides is too small to prove effectual. It might be presumed 

 that an increase of the amount of amides had taken place and this circumstance 

 disguised by the amides being translocated to the branches, but this possibility 

 is contradicted by the result, found in II 3, which proves that injury of the isolated 

 buds has no influence upon the formation of amides. The buds of I 3, which were 

 exposed to darkness but otherwise were treated as those in II 3, show on the con- 



