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liary a considerable increase of amides, which must consequently be owing to the 

 exposure to darkness. Ill 7 do not show any increase of the amides, it is true, but 

 in this case a translocation of the amides to the branches is possible; and besides, 

 the transpiration has doubtlessly been retarded, the buds being covered with paper, 

 a hypothesis implied by their heightened weight. The increased amount of water 

 proves the result in the table to be too small. Supposing the buds, uncovered, had 

 had the same weight as those of 6, a considerable amount of amides is found (cfr. 

 the numbers in brackets). It is left to be proved whether the accumulation of the 

 amides is only to be referred to the retardation of the synthesis of the proteids, 

 produced by the exposure to darkness; it is however probable that the carbo- 

 hydrates, physiologically effective, which were originally present in the buds, have 

 been used by the respiratory process, so that lack of such compounds prevents 

 the synthesis taking place. 



The experiments on etherization show that small doses (01 c.c. ether p. liter) 

 produce an increase of the amount af amides both in buds, exposed to light (cfr. 

 difference between III 4 and II 3), and in buds, exposed to darkness (cfr. Ill 3 -=- III 2). 

 Larger doses (05 c. c. ether p. liter) have also retarded the synthesis of proteids of 

 buds in light, although in a less degree (III 5). It is questionable whether the ether 

 has caused the arresting process or whether it is a case of katabolism occasioned 

 by the death of the plant. The latter alternative is probable, partly because the 

 buds at the end of the experiment were discoloured, partly because a reduction of 

 the amides has been affected by the same dose, used in darkness, and in this case 

 the buds were unhurt after the exposure. The same dose having thus pi-oduced 

 dissimilar effect on the lightened and darkened buds, this phenomenon may be 

 explained by the circumstance that the temperature, to which the lightened buds 

 had been exposed, was somewhat higher than that in which the darkened ones 

 remained, but possibly also by the fact that light may increase the effect of ether. 

 Unfortunately I have not been able through III 3 and III 4 to arrive at any in- 

 formation concerning the latter question, as we have no information as to the 

 effects of doses which are slightly smaller or slightly larger than that used in 

 this case. 



Darkened buds which have been exposed for two days to larger doses have 

 died; the smallest dose that proved deadly, used in I, produced a small decrease 

 of amides, which fact indicates that the toxic effect did not set in till late; if the 

 toxic effect had set in immediately, considerable increase of amides would have 

 taken place. 



