VETERINARY SCIENCE AND PRACTICE, 991 



The disease can be transmitted to tlie guinea pig by ingestion, 

 inhalation, or hypodermic injection. The white rat succumbs after 

 about 30 hours to inoculation of pure cultures of this organism. In 

 the dog the results of an inoculation are less constant. Intravenous 

 inoculations are ahvaj's fatal within from 30 to 72 hours. Frogs 

 maintained at a temperature of from 25 to 28° C. succumb rapidly to 

 hypodermic or peritoneal inoculations. 



The repeated passing of the organism through animals increases its 

 virulence. In the case of the rabbit it was observed that repeated pas- 

 sages through the rab})it increased the virulence of the organism for 

 the rabbit, but did not alter its virulence toward the pigeon to a notice- 

 able degree. Repeated doses of sterile cultures produced an innnunity 

 through a hypodermic inoculation in the rabbit and guinea pig. 



Tests of various antiseptics, F. T. Bioletti {C<(I!f<*i'n!<i Sta. 

 Rpt. 1898^ pjp. 170-17S). — An analysis was made of a substance called 

 Puriline, for which high claims were made as to its antiseptic power. 

 It was found not to contain chlorin and bromin, as claimed >)y the 

 manufacturers. Puriline was compared with a num1)er of other anti- 

 septics, such as bittern water, aluminum sulphate, aluminum chlorid, 

 boracic acid, and calcium chlorid. Various quantities of these antisep- 

 tics were placed in sterilized flasks containing ordinary beef bouillon, 

 and they were then inoculated with putrefactive bacteria. The slight 

 antiseptic power of Puriline was found to ))e due largelv to the alumi- 

 num chlorid which it contained. This sul)stance, however, was found 

 to be far too expensive for practical application. 



Sea water, to which aluminum sulphate was added in the proportion 

 of 55 lbs. to 100 gal., was found to be an etfective antiseptic in pre- 

 venting decomposition, when used in the ratio of IS gal. to lOo gal. 

 of ordinary sea water. A test of an extract of California laurel 

 [UmhtUnJaria californica) showed that this substance had no appre- 

 ciable effect in checking the growth of bacteria. 



Alcohol fumes as a disinfectant, ^V. von Brunn {CentlA. Bakt. a. 

 Bi,'., 1 Abt, 28 {1900), So. 10-11, pp. 309-315, fig ><. ;g).— The author 

 conducted a number of experiments to determine the disinfectant 

 power of alcohol fumes upon anthrax bacillus. The material used in 

 these experiments came from agar cultures from three sources. The 

 anthrax bacillus was dried upon the surface of silk threads and in this 

 condition exposed to the action of fumes from alcohols of different 

 concentration. It was found as a result of these experiments that 10 

 per cent alcohol has but little effect upon the anthrax bacillus, while a 

 25 and 50 per cent alcohol gave off' fumes which were considerably 

 more active. The best results were obtained from the fumes of a 75 

 per cent alcohol. When 95 per cent alcohol was used it was found that 

 the fumes had no effect on the anthrax liacillus. These results indi- 

 cate that alcohol fumes are most eff'ective as disinfectants when mixed 



