iioo PROTEIN METABOLISM [pt. m 



exceeds my value of o-o6 mgm. for amniotic and allantoic liquids 

 and embryo. Such discrepancies seem to be inevitable in the first 

 approximations, and can only be abolished by further work. Kamei's 

 figures for the non-protein nitrogen fractions are not easy to interpret. 



Table 145. Concentration of constituents in the amniotic liquid of 

 the chick. {Milligrams °l^.) 



Fiske & Boyden Kamei Targonski 



1 ^ c 



c o • Ji "sc -s^a o i; -5 



1 ^ -I is 2^ g'c I I c gc 



I -Sc % Is g2| §2 I S I I §2 



Day h D& h hS 2c^ Zc < D h £ Zc 



2 — — — — — — — — — — — 



3— ___ — —_ — ___ 



4— __ _ _______ 



5— __________ 



6 — o-io — — — — — — — — — 



7 2-9 — — — — —— — — — — 



8— __________ 



9 — o-io 1 1-9 33-1 1-5 0-6 0-8 3-4 — — — 



10 — 0-13 — — — — — — 17-0 — — 



11 7-5 o-ii — — — — — — — — — 



12 156 _______ 40-5 — — 



13 2450 — — — ^ — — — — — — — 



14 — — 3184 37-1 1-3 3-5 1-9 3-4 58-0 — — 



15 _ __________ 



16 1500 0-17 _ _ _ _ _ _ 5450 5200 250 



17 — — 2003 30-3 4-4 2-2 1-7 14-3 _ _ _ 

 18— _______ 5300 _ — 



19 _ __________ 



20 — — — — — — — — — — — 



The effect of the nitrogen metabolism on the amniotic fluid of the 

 chick has been investigated by Fiske & Boyden; Targonski, and Kamei. 

 As Table 145 shows, the total nitrogen concentration of the amniotic 

 liquid rises very slowly until the 12th day, at which time it increases 

 in prodigious strides, but, as Fiske & Boyden put it, "this merely 

 serves to give a quantitative aspect" to the fact, discovered by Hirota 

 and Fiilleborn, that in the last half of the 2nd week of incubation a 

 communication is established between the albumen-sac and the 

 amnion at the site of the sero-amniotic junction. As the data of 

 Targonski and of Kamei in Table 145 show, this large increase of 

 nitrogen is almost entirely due to protein. As for the uric acid, its 

 concentration does not increase proportionately to the growth of the 



