62 



F. EDMUND HUNTER, JR. 



iodoacetamide produce swelling, but it resembles that seen with phosphate 

 more than that due to ascorbate. An interesting observation from these 

 experiments is that very low ^-hydroxymercuribenzoate concentrations 

 produce swelling after a lag period, while 60-100 /xM levels have a three- 



0.5 



0.4 



0.3 



0.2 



0.1 



CONTROL 



p-OH-MERCURIBENZOATE 



10 



20 



30 40 



MINUTES 



50 



60 



Fig. 13. Absorbancy changes due to different concentrations of /)-hydroxy- 

 mercuribenzoate. High concentrations show a three-phase curve. 



20 30 40 



MINUTES 



50 



60 



Fig. 14. Ascorbate added with or after p-hydroxymercuribenzoate produces 

 fairly typical lysis. 



phase effect — first a rapid fall in absorbancy, then a definite plateau for 

 about 10 min., then a further fall (Fig. 13). This may indicate two sites of 

 action or an immediate and a delayed effect from a single site of action. 

 Pyruvate seems to inhibit the initial phase with little effect on the second 

 fall. 



