ASCORBATE-INDUCED LYSIS OF ISOLATED MITOCHONDRIA 69 



might be due to stimulation of an endogenous substrate or something. These 

 experiments do bring to mind that Ottolenghi in his experiments on lipid per- 

 oxidation by mitochondria found that ascorbate was oxidized only until the lipid 

 was used up. Then oxidation stopped and ascorbate was not used. It probably 

 depends on the ratio of ascorbate to mitochondria ; if you use an excess of ascorbate 

 most of it will be there when your experinient is finished. 



