Integrated Oxidations in Isolated Mitochondria 



J. B. Chappell* 

 Department of Biochemistry, Uuirersity of Cambridge, England 



It appears that, in intact mitochondria, a straightforward reduction 

 of pyridine nucleotide by substrate, followed by reoxidation of the reduced 

 nucleotide by the cytochrome system does not occur without complications 



Ag-AgCI 



0-5M KCI 



Pt electrode 



Perspex disc 



Tlea' 



O' ring supporting 

 membrane 



-Support 

 for vessel 



1cm 



Fig. I. The Clark oxygen electrode adapted for following mitochondrial 

 respiration. 



arising because of subsequent events at the substrate level. This paper 

 represents an attempt to justify this statement. All the investigations 

 reported have been performed w-ith the Clark oxygen electrode set up as 



* Present address : TJie Johnsnii Foundation, University of Pennsylvania, 

 Philadelphia, U.S.A. 



