FUN'CTIOX OF FLAVOEXZYMES IN ELECTRON TRANSPORT 



O/ 



the maintenance of adequate levels of high-energy intermediates needed 

 for the endergonic reduction of DPN by succinate. 



5x10 



M dmitrophenol 



Fig. II. Inhibition of succinate-linked reduction of acetoacetate by 2,4-dinitro- 

 phenol (Azzone, Ernster, and Weinbach, unpublished). Each flask contained: 

 mitochondria from 400 mg. liver, 10 mM succinate, 5 mM acetoacetate, and 

 dinitrophenol as indicated, in a final volume of 2 ml. Other conditions as in Fig. 10. 

 Time of incubation, 20 min. 



TABLE VIII 



Influence of Mg~~ .\nd of Terminal Phosphate Acceptor on the 

 Succinate-linked Reduction of Acetoacet.ate 



(Azzone, Ernster and Weinbach, unpublished) 



Each Warburg flask contained: mitochondria from 250 mg. liver, 20 niM gly- 

 cylglycine bufi^er, pH 7-5, 62 mM sucrose, 50 mM KCl, 10 mM succinate, 5 mM 

 acetoacetate, 15 mM Pj, and where indicated, 15 mM AMP, i mM .\TP, 15 mM 

 glucose, and an excess of yeast hexokinase, in a final volume of 2 ml. Gas phase, 

 air; in centre well, 02 ml. 2 M KOH; temperature, 30 . Time of incubation, 

 16 min. 



Additions 



8 niM Mg 



Xo Mg- 



— JAcAc Oxygen „ , „ — JAcAc Oxygen „,„ 



(/xmoles) (/tatoms) ' (/^tmoles) (/xatoms) 



8-7 

 II-3 



1-79 



This concept was further emphasized by the findings recorded in 

 Table \TII. It has been demonstrated first by Baltscheffsky [44] that 

 incubation of rat liver mitochondria in the absence of ^Ig^~ abolishes the 



