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MARTIN KLINGENBERG 



of succinate a higher ATP-level is formed than with dinitrophenol. How- 

 ever, it is remarkable that also in the presence of succinate appreciable 

 amounts of ADP and AMP are found together with inorganic phosphate. 



100 125 

 Fractions 



Fig. I. Anion exchange chromatograms (Dowex 2) of perchloric acid extracts 

 of pigeon-breast muscle mitochondria. Before extraction the mitochondria were 

 incubated for 60 sec. in about 15 ml. of the reaction medium, containing 0-3 M 

 sucrose, 10 mivi triethanolamine-HCl-buffer, pH 72, 25". This medium was 

 oxygen (i atm.) saturated. 



Chromatogram a. Mitochondria (47 mg. protein) incubated under addition 

 of 4 mM succinate. 



Chromatogram b. Mitochondria (47 mg. protein) incubated under addition 

 of o • I mM dinitrophenol. 



In a first attempt to correlate functional states of the three major 

 mitochondrial nucleotide systems, the phosphorylation state of the adenine 



